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Anti-Bacillus thuringiensis CRY1Ab Toxin抗体(ab51586)

Submit a review Q&A (2)References (4)

Key features and details

  • Rabbit polyclonal to Bacillus thuringiensis CRY1Ab Toxin
  • Suitable for: IP, WB, ELISA
  • Isotype: IgG

概述

  • 产品名称

    Anti-Bacillus thuringiensis CRY1Ab Toxin抗体

  • 描述

    兔多克隆抗体to Bacillus thuringiensis CRY1Ab Toxin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, WB, ELISAmore details

  • 种属反应性

    与反应: Bacillus thuringiensis

  • 免疫原

    Full length native protein (purified) corresponding to Bacillus thuringiensis CRY1Ab Toxin.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51586于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
ELISA
1/500 - 1/800.
说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
ELISA
1/500 - 1/800.

靶标

  • 相关性

    CRY1A belongs to the delta endotoxin family and is one of the most studied insecticidal proteins produced by Bacillus thuringiensis. The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat. The toxic segment of the protein is located in the N-terminus. Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

  • 别名

    • 130 kDa crystal protein antibody
    • bt2 antibody
    • cry 1 2 antibody
    • cry1A(b) antibody
    • cryIA(b) antibody
    • cryIC1 antibody
    • Crystaline entomocidal protoxin antibody
    • Insecticidal delta endotoxin CryIA(b) antibody
    • Pesticidal crystal protein cry1Ab antibody
    see all

文献 (4)

发表研究结果有使用 ab51586？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab51586 被引用在 4 文献中.

  • Ding L  et al. Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm. PLoS One 12:e0178754 (2017). PubMed: 28582405
  • Rocha LO  et al. Multi-Method Approach for Characterizing the Interaction between Fusarium verticillioides and Bacillus thuringiensis Subsp. Kurstaki. PLoS One 9:e92189 (2014). IF . PubMed: 24739804
  • Gu K  et al. Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis d-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus). Biotechnol Biofuels 7:68 (2014). WB ; Rice . PubMed: 24808924
  • Yang H  et al. Weak transcription of the cry1Ac gene in nonsporulating Bacillus thuringiensis cells. Appl Environ Microbiol 78:6466-74 (2012). WB . PubMed: 22773626

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Good morning,

I would like to know if you can produce two monoclonal antibodies antidobies, but that these recognize different epitopes of the cry1Ab toxin.



Thanks.

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Answer

Thank you for contacting us and your interest in our products.

We currently have two antibodies available in our catalogue directed against the cry1Ab toxin. One is a rabbit polyclonal antibody (ab51586) raised against purified Bacillus thuringiensis CRY1Ab protein, the other a mouse monoclonal (ab113679) raised against the Bacillus thuringiensis Cry1Ab Toxin /Cry1Ac fusion protein.

As the rabbit polyclonal antibody ab51586 is raised against the whole length protein we would not be able to say which part of the proteins is recognised. However, the mouse monoclonal (ab113679) has been raised against a peptide taken from within the residues 400 to 440 of Bacillus thuringiensis CRY1Ab Toxin.

Unfortunately, we are not able to provide custom antibody synthesis at present, but our sister company Epitomics may be able to help. The specialize in making rabbit monoclonal antibodies. They can be reached on the following email: mailto:service@epitomics.com

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

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Question

The cat no is Ab51586.

Application is Elisa .

Regards

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Answer

Thank you for your reply.

I am sorry to confirm that at this time we haven’t tested ab51586Bacillus thuringiensis CRY1Ab Toxin antibodyfor detection of fusion cotton gene.

I'm sorry I couldn't give you positive news on this occasion. If you have any further questions,please do not hesitate to contact us.

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