Anti-BACH1.3抗体[OTI4E11] (ab128486)
Key features and details
- Mouse monoclonal [OTI4E11] to BACH1.3
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-BACH1.3抗体[OTI4E11]
参阅全部 BACH1.3 一抗 -
描述
小鼠单克隆抗体[OTI4E11] to BACH1.3 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human BACH1.3 aa 1-736. produced in HEK293T cells (NP_996749).
Sequence:MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRS VLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHC QKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIH ASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKG FWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQ RTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISL GDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKL TPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSF LISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE
Database link: O14867 -
阳性对照
- WB: HEK-293T cells transfected with BACH1.3. IHC-P: Human bladder carcinoma and tonsil tissues. Flow Cyt (Intra): BACH1 transfected HEK-293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI4E11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 82 kDa.
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IHC-P |
1/150.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/2000. Predicted molecular weight: 82 kDa. |
IHC-P
1/150. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK. -
序列相似性
Belongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 571 Human
- Omim: 602751 Human
- SwissProt: O14867 Human
- Unigene: 154276 Human
- Unigene: 721915 Human
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别名
- BACH 1 antibody
- BACH 1.3 antibody
- Bach1 antibody
see all
图片
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All lanes : Anti-BACH1.3 antibody [OTI4E11] (ab128486) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY BACH1.3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
Paraffin-embedded human bladder carcinoma tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)
Paraffin-embedded human tonsil tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY BACH1.3 (Red) or empty vector control plasmid (Blue) stained for BACH1.3 using ab128486 at 1/100 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab128486 被引用在 2 文献中.
- Liu S et al. Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression. Aging (Albany NY) 13:17274-17284 (2021). PubMed: 34191748
- Huang X et al. Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine. Theranostics 8:3366-3379 (2018). PubMed: 29930735