Anti-BACE1抗体(ab2077)
Key features and details
- Rabbit polyclonal to BACE1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BACE1抗体
参阅全部 BACE1 一抗 -
描述
兔多克隆抗体to BACE1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
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阳性对照
- WB: Human A431, A549, Caco-2, Daudi, HeLa, K562, Jurkat, SK-N-SH, THP-1 and brain tissue lysates. Mouse: 3T3/NIH cell lysate. IHC-P: Mouse brain tissue. ICC: Mouse 3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
Primary antibody说明
Beta-site APP Cleaving Enzyme. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa. We recommend overnight incubation at 4C and using 5% skim milk to block.
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| ICC |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa. We recommend overnight incubation at 4C and using 5% skim milk to block. |
|
ICC
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
组织特异性
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
序列相似性
Belongs to the peptidase A1 family. -
结构域
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- Unigene: 504003 Human
- Unigene: 24044 Mouse
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别名
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
图片
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Western blot - Anti-BACE1 antibody (ab2077)All lanes :
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 4 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 6 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 8 : SK-N-SH (Human neuroblastoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 9 : THP-1 (Human monocytic leukemia cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lysates/proteins at 15 µg per lane.
Blocking peptides at 1 µg/ml per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Additional bands at: 65 kDa (possible glycosylated form)10% gel.
Running conditions: 130v for 2 hours.
Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).
Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.
Primary antibody incubation: Room temperature for 1 hour.
Secondary antibody incubation: Room temperature for 1 hour.
Washing conditions: 15 mL TSBT, 3 x 10 minutes.
Exposure: ECL solution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody (ab2077)Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab2077 at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry - Anti-BACE1 antibody (ab2077)Immunocytochemical analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on NIH/3T3 cells.
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Immunocytochemistry - Anti-BACE1 antibody (ab2077)Immunocytochemical analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 10 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. -
Western blot - Anti-BACE1 antibody (ab2077)All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml
Lane 1 : Human brain tissue lysate with absence of blocking peptide
Lane 2 : Human brain tissue
lysate with BACE1 peptide (ab7883)
Lane 3 : Mouse 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?Incubate the antibody for 1 hour at room temperature in 5% NFDM/TBST.
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Immunocytochemistry - Anti-BACE1 antibody (ab2077)Image courtesy of an anonymous Abreview.Immunocytochemical analysis of D54MG (human glioblastoma cell line) cells labeling BACE1 with ab2077.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (91)
ab2077 被引用在 91 文献中.
- Kirschenbaum D et al. Whole-brain microscopy reveals distinct temporal and spatial efficacy of anti-Aβ therapies. EMBO Mol Med 15:e16789 (2023). PubMed: 36382364
- Wen QX et al. AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice. Traffic 24:20-33 (2023). PubMed: 36412210
- Kwon YS et al. Oleracone F Alleviates Cognitive Impairment and Neuropathology in APPswe/PSEN1dE9 Mice by Reducing the Expression of Vascular Cell Adhesion Molecule and Leukocyte Adhesion to Brain Vascular Endothelial Cells. Int J Mol Sci 24:N/A (2023). PubMed: 36768379
- Liu Y et al. DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation. Brain Sci 13:N/A (2023). PubMed: 37239217
- Iyaswamy A et al. Theranostic F-SLOH mitigates Alzheimer's disease pathology involving TFEB and ameliorates cognitive functions in Alzheimer's disease models. Redox Biol 51:102280 (2022). PubMed: 35286997