Anti-B4GALT3抗体(ab74825)
Key features and details
- Rabbit polyclonal to B4GALT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-B4GALT3抗体
参阅全部 B4GALT3 一抗 -
描述
兔多克隆抗体to B4GALT3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthesized peptide derived from an internal region of human B4GALT3
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阳性对照
- WB: extracts from HepG2 cells, HeLa cells and Jurkat cells IHC-P: human liver carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74825于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
|
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. -
组织特异性
Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 7 family. -
细胞定位
Golgi apparatus > Golgi stack membrane. Trans cisternae of Golgi stack. - Information by UniProt
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数据库链接
- Entrez Gene: 8702 Human
- Entrez Gene: 8703 Human
- Entrez Gene: 8704 Human
- Entrez Gene: 53418 Mouse
- Entrez Gene: 56375 Mouse
- Entrez Gene: 57370 Mouse
- Entrez Gene: 303923 Rat
- Entrez Gene: 494342 Rat
see all -
别名
- 4-galactosyltransferase 3 antibody
- 4-galactosyltransferase antibody
- 4-GalTase 3 antibody
see all
图片
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Western blot - Anti-B4GALT3 antibody (ab74825)All lanes : Anti-B4GALT3 antibody (ab74825) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HeLa cells
Lane 3 : extracts from Jurkat cells
Lane 4 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT3 antibody (ab74825)ab74825, at a dilution of 1/50, staining B4GALT3 in paraffin embedded human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab74825 被引用在 1 文献中.
- Zhou H et al. B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1. Cell Death Dis 4:e654 (2013). WB ; Human . PubMed: 23744354