Anti-B4GALT1抗体(ab121326)
Key features and details
- Rabbit polyclonal to B4GALT1
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-B4GALT1抗体 -
描述
兔多克隆抗体to B4GALT1 -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 50-163 of Human B4GALT1 (P15291).
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阳性对照
- IHC-P: Human placenta, prostate, and testis tissue sections; WB: HeLa cell lysate; ICC: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121326于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. -
组织特异性
Ubiquitously expressed, but at very low levels in fetal and adult brain. -
通路
Protein modification; protein glycosylation. -
疾病相关
Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. -
序列相似性
Belongs to the glycosyltransferase 7 family. -
翻译后修饰
The soluble form derives from the membrane forms by proteolytic processing. -
细胞定位
Golgi apparatus > Golgi stack membrane. Cell membrane. Cell surface. Found in trans cisternae of Golgi; Golgi apparatus > Golgi stack membrane. Found in trans cisternae of Golgi and Secreted. Soluble form found in body fluids. - Information by UniProt
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数据库链接
- Entrez Gene: 2683 Human
- Omim: 137060 Human
- SwissProt: P15291 Human
- Unigene: 272011 Human
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别名
- 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody
- 4-galactosyltransferase 1 antibody
- 4-GalTase 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-B4GALT1 antibody (ab121326)Immunocytochemistry analysis of A431 (human epidermoid carcinoma) cells labelling B4GALT1 with ab121326 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-B4GALT1 antibody (ab121326)All lanes : Anti-B4GALT1 antibody (ab121326) at 2 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysateLoading control: Anti-HSP90B1
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (ab121326)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab121326 被引用在 8 文献中.
- Cui Y et al. B4GALT1 promotes immune escape by regulating the expression of PD-L1 at multiple levels in lung adenocarcinoma. J Exp Clin Cancer Res 42:146 (2023). PubMed: 37303063
- Chen P et al. Single-Cell and Spatial Transcriptomics Decodes Wharton's Jelly-Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures. Adv Sci (Weinh) 10:e2204786 (2023). PubMed: 36504438
- De Vitis C et al. B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis. Int J Mol Sci 23:N/A (2022). PubMed: 36499368
- Ren Z et al. High expression of B4GALT1 is associated with poor prognosis in acute myeloid leukemia. Front Genet 13:882004 (2022). PubMed: 36568388
- Chen Y et al. Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. Cancer Lett 500:228-243 (2021). PubMed: 33309857
- Srivastava T et al. Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes. Am J Physiol Renal Physiol 319:F312-F322 (2020). PubMed: 32628542
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611
- Wu J et al. ZFX modulates the growth of human leukemic cells via B4GALT1. Acta Biochim Biophys Sin (Shanghai) 48:1120-1127 (2016). PubMed: 27797721