Anti-AZI1抗体(ab99379)
Key features and details
- Rabbit polyclonal to AZI1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AZI1抗体
参阅全部 AZI1 一抗 -
描述
兔多克隆抗体to AZI1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human AZI1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. EAW89632.1
Database link: Q9UPN4 -
阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99379于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
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| IP | (1) |
Use at 2-5 µg/mg of lysate.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 122 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
May play a role in spermatogenesis. -
序列相似性
Contains 1 IQ domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 22994 Human
- Omim: 613479 Human
- SwissProt: Q9UPN4 Human
- Unigene: 514578 Human
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别名
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
see all
图片
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Western blot - Anti-AZI1 antibody (ab99379)All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 122 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunoprecipitation - Anti-AZI1 antibody (ab99379)Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab99379 被引用在 7 文献中.
- Ando K et al. CEP131 Abrogates CHK1 Inhibitor-Induced Replication Defects and Is Associated with Unfavorable Outcome in Neuroblastoma. J Oncol 2020:2752417 (2020). PubMed: 33014050
- Shang Z et al. USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis. J Biol Chem 294:9844-9857 (2019). PubMed: 31073027
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Li X et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . PubMed: 28361952
- Airik R et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS One 11:e0156081 (2016). PubMed: 27224062
- Staples CJ et al. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability. J Cell Sci 127:2910-9 (2014). WB . PubMed: 24816561
- Staples CJ et al. The centriolar satellite protein Cep131 is important for genome stability. J Cell Sci 125:4770-9 (2012). PubMed: 22797915