AZdye 568 Anti-mNeonGreen antibody [1E2] - FluoTag®-X2

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mNeonGreen antibody [1E2] - FluoTag®-X2 (AB325303)

PFA-fixed Cos7 cells expressing a TOM70-nfmNeonGreen fusion protein (nf : non-fluorescent) were stained with ab325303 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325303. (B) False color representation of the image shown in A is presented in red (coloring according to the excitation wavelength of the employed fluorophore). (C) The corresponding DAPI signal of the depicted section. (D) Merge of A and C. False color representation of A in red and C in blue.

• Other

Supplier Data

Other - AZdye 568 Anti-mNeonGreen antibody [1E2] - FluoTag®-X2 (AB325303)

FluoTag®

FluoTag® reagents are alpaca-derived single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores at known positions.

Depending on the specific target protein, they are available in up to three variants :
• FluoTag®-Q : A single sdAb conjugated to a single fluorophore, ideal for quantitative readout.
• FluoTag®-X2 : A single sdAb conjugated to two fluorophores, our standard variant.
• FluoTag®-X4 : A blend of two different FluoTags®-X2 that bind to a single target. FluoTag®-X4 reagents effectively label the target (T) with 4 fluorophores in total, resulting in brighter signals.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mNeonGreen antibody [1E2] - FluoTag®-X2 (AB325303)

Immunostaining of PFA-fixed Cos7 cells expressing a TOM70-mNeonGreen reporter protein with ab325303 at a 1/500 dilution. The mNeonGreen signal is represented in green, the corresponding FluoTag® signal is represented in red and the merge of both channels is represented in yellow. Nuclei were visualized by DAPI staining (blue).