Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组

重组Anti-Axl抗体[7E10] (ab130218)

Submit a review Submit a question
Western blot - Anti-Axl antibody [7E10] (ab130218)
  • Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] (ab130218)
  • Flow Cytometry - Anti-Axl antibody [7E10] (ab130218)
  • Anti-Axl antibody [7E10] (ab130218)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7E10] to Axl
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

概述

  • 产品名称

    Anti-Axl抗体[7E10]
    参阅全部 Axl 一抗

  • 描述

    小鼠单克隆抗体[7E10] to Axl

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt: HeLa cells. ICC/IF: HeLa cells. WB: DU145, NCI-H1299 and HeLa whole cell lysates.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab130218于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 0.974 µg/ml.
WB
Use a concentration of 1.947 µg/ml. Detects a band of approximately 80, 140 kDa (predicted molecular weight: 98 kDa).
ICC/IF
Use a concentration of 7.788 µg/ml.
说明
Flow Cyt
Use a concentration of 0.974 µg/ml.
WB
Use a concentration of 1.947 µg/ml. Detects a band of approximately 80, 140 kDa (predicted molecular weight: 98 kDa).
ICC/IF
Use a concentration of 7.788 µg/ml.

靶标

  • 功能

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.

  • 组织特异性

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Adhesion related kinase antibody
      • AI323647 antibody
      • Ark antibody
      • Axl antibody
      • AXL oncogene antibody
      • AXL receptor tyrosine kinase antibody
      • AXL transforming gene antibody
      • AXL transforming sequence/gene antibody
      • EC 2.7.10.1 antibody
      • JTK11 antibody
      • Oncogene AXL antibody
      • Tyro7 antibody
      • Tyrosine protein kinase receptor UFO antibody
      • Tyrosine-protein kinase receptor UFO antibody
      • UFO antibody
      • UFO_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Axl antibody [7E10] (ab130218)
      Western blot - Anti-Axl antibody [7E10] (ab130218)
      All lanes : Anti-Axl antibody [7E10] (ab130218) at 1.947 µg/ml

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 2 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate
      Lane 3 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 98 kDa
      Observed band size: 140,80 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      The molecular weight observed was consistant with the literature (PMID: 19541935).

      Negative control: Jurkat (PMID: 28423548).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] (ab130218)
      Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] (ab130218)

      Immunofluorescent analysis of 4% methanol-fixed, 0.1% Triton X-100 permeabilized NCI-H1299 cells labeling Axl with ab130218 at 7.788 µg/mL, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NCI-H1299 cell line.

      Negative control: Jurkat (PMID:28423548).

      The nuclear counterstain is DAPI (blue). Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab130218 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
      -ve control 2: ab179513 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    • Flow Cytometry - Anti-Axl antibody [7E10] (ab130218)
      Flow Cytometry - Anti-Axl antibody [7E10] (ab130218)

      Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left panel) / HeLa (Human cervix adenocarcinoma epithelial cell, Right panel) labeling Axl using Anti-Axl antibody [7E10] (ab130218) at 0.974 µg/mL. Mouse monoclonal IgG isotype control (Black). The unlabeled control is cells without incubation with primary antibody or secondary antibody (Blue).

      Negative control: Jurkat.

      Gated on viable cells.

    • Anti-Axl antibody [7E10] (ab130218)
      Anti-Axl antibody [7E10] (ab130218)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab130218？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab130218 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab130218.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com