Anti-Axin 2抗体(ab107613)
Key features and details
- Rabbit polyclonal to Axin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Axin 2抗体
参阅全部 Axin 2 一抗 -
描述
兔多克隆抗体to Axin 2 -
宿主
Rabbit -
特异性
ab107613 antibody is predicted to not cross-react with Axin 1. At least four isoforms of Axin 2 are known to exist; this antibody will recognize all four. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human Axin 2 (C terminal). The immunogen is located within amino acids 780 - 830 of Axin 2.
-
阳性对照
- WB: Mouse lung tissue lysate. IHC-P: Mouse lung and colon tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab107613于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. |
靶标
-
功能
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. -
组织特异性
Expressed in brain and lymphoblast. -
疾病相关
Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
序列相似性
Contains 1 DIX domain.
Contains 1 RGS domain. -
结构域
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
翻译后修饰
Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 12006 Mouse
- SwissProt: O88566 Mouse
- Unigene: 71710 Mouse
-
别名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
图片
-
Western blot - Anti-Axin 2 antibody (ab107613)Anti-Axin 2 antibody (ab107613) at 1 µg/ml + Mouse lung tissue lysate at 15 µg
Predicted band size: 94 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse lung tissue staining Axin 2 with ab107613 at 20 µg/mL.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse lung tissue labeling Axin 2 with Anti-Axin 2 antibody (ab107613) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)This image is courtesy of an anonymous Abreviewab107613 staining Axin 2 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab107613 被引用在 2 文献中.
- Rauner M et al. Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nat Metab 1:111-124 (2019). PubMed: 30886999
- Ouyang J et al. The role of aplysia ras homolog I in colon cancer cell invasion and adhesion. Exp Ther Med 14:5193-5199 (2017). PubMed: 29201236