Anti-Avian Influenza Nucleoprotein抗体(ab25921)
Key features and details
- Rabbit polyclonal to Avian Influenza Nucleoprotein
- Suitable for: WB
- Reacts with: Influenza A
- Isotype: IgG
概述
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产品名称
Anti-Avian Influenza Nucleoprotein抗体
参阅全部 Avian Influenza Nucleoprotein 一抗 -
描述
兔多克隆抗体to Avian Influenza Nucleoprotein -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Influenza A -
免疫原
Synthetic peptide corresponding to Avian Influenza Nucleoprotein aa 58-77.
Sequence:KLSDYEGRLIQNSITIERMV
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阳性对照
- Recombinant fusion protein of avian flu nucleoprotein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25921于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
Please note from the image legend that a recombinant fusion protein was used to generate the western blot image.
The molecular weight shown is the molecular weight of the fusion protein (e.g nucleoprotein amino acids 58-77 + fusion protein) not the Avian Influenza Nucleoprotein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus -
细胞定位
Nuclear -
数据库链接
- SwissProt: P68881 Influenza A
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别名
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
图片
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Western blot - Anti-Avian Influenza Nucleoprotein antibody (ab25921)All lanes : Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/ml
Lane 1 : Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg
Lane 2 : Fusion partner without these amino acids
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 59 kDa, 66 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (0)
ab25921 尚未被引用在任何文献中。
