AB25921

Anti-Avian Influenza Nucleoprotein抗体

Anti-Avian Influenza Nucleoprotein antibody

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Polyclonal NCAP antibody. Suitable for WB and reacts with Recombinant full length protein - Influenza A samples.

查看别名

Nucleoprotein, Nucleocapsid protein, Protein N, NP

1 Images

Unknown

Western blot - Anti-Avian Influenza Nucleoprotein antibody (AB25921)

All lanes:

Western blot - Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/mL

Lane 1:

Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg

Lane 2:

Fusion partner without these amino acids

Observed band size: 31 kDa,56 kDa,59 kDa,66 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Influenza A

应用

applications

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Avian Influenza Nucleoprotein also known as NP is a structural protein found in influenza viruses particularly avian strains. It functions mechanically as a part of the ribonucleoprotein (RNP) complex encapsulating the viral RNA in a helical structure therefore facilitating its replication and transcription. The molecular weight of the nucleoprotein is approximately 56 kDa. It expresses predominantly within the host cell's cytoplasm participating in viral assembly and eventually packaging.

Biological function summary

The nucleoprotein functions as an important player in viral replication and interaction with host cell machinery. Within the RNP complex it binds to viral RNA and polymerase subunits ensuring the stability of viral genome replication. Additionally the nucleoprotein interacts with host nuclear import and export pathways to shuttle viral components across the nuclear membrane efficiently important for viral propagation within host cells.

Pathways

Avian Influenza Nucleoprotein influences viral RNA synthesis and export pathways. It associates with viral polymerase proteins such as PA PB1 and PB2 during the viral RNA replication process. Moreover its interaction with nuclear export factors like exportin-1 (CRM1) integrates it into the host's nuclear export pathway facilitating the movement of viral RNA from nucleus to cytoplasm which is essential for the completion of the viral life cycle.

Avian Influenza Nucleoprotein is central to the pathogenicity of avian influenza infections which can result in avian influenza virus (AIV) outbreaks. This relationship with AIV highlights its importance in zoonotic transmission and potential emergence of avian influenza in humans. Its association with proteins involved in the immune response such as interferon-inducible proteins also highlights its role in modulating host immune evasion a factor that complicates the control of influenza outbreaks.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

See full target information NP

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Avian Influenza Nucleoprotein抗体

Anti-Avian Influenza Nucleoprotein antibody

Western blot - Anti-Avian Influenza Nucleoprotein antibody (AB25921)