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Anti-Avian甲型流感Neuraminidase抗体(ab21305)

Submit a review Q&A (3)References (3)
Western blot - Anti-Avian Influenza A Neuraminidase antibody (ab21305)
  • ELISA - Anti-Avian Influenza A Neuraminidase antibody (ab21305)

Key features and details

  • Rabbit polyclonal to Avian Influenza A Neuraminidase
  • Suitable for: ELISA, WB
  • Reacts with: Influenza A
  • Isotype: IgG

概述

  • 产品名称

    Anti-Avian甲型流感Neuraminidase抗体
    参阅全部 Avian Influenza A Neuraminidase 一抗

  • 描述

    兔多克隆抗体to Avian甲型流感Neuraminidase

  • 宿主

    Rabbit

  • 特异性

    ab21305 can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Influenza A

  • 免疫原

    Synthetic peptide corresponding to 16 amino acids in the middle of the Neuraminidase protein. Efforts were made to use relatively conserved regions as the antigen.

  • 阳性对照

    • WB: Avian Influenza Neuraminidase recombinant protein. ELISA: Avian Influenza Neuraminidase recombinant protein.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab21305于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use a concentration of 1 µg/ml.

Detects 10 ng of free peptide.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
说明
ELISA
Use a concentration of 1 µg/ml.

Detects 10 ng of free peptide.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

靶标

  • 相关性

    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.

  • 细胞定位

    Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).

  • 数据库链接

    • 别名

      • NA antibody
      • Neuraminidase antibody

    图片

    • Western blot - Anti-Avian Influenza A Neuraminidase antibody (ab21305)
      Western blot - Anti-Avian Influenza A Neuraminidase antibody (ab21305)
      All lanes : Anti-Avian Influenza A Neuraminidase antibody (ab21305) at 1 µg/ml

      Lane 1 : 50 ng of Avian Influenza Neuraminidase recombinant protein
      Lane 2 : 100 ng of Avian Influenza Neuraminidase recombinant protein

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 51 kDa



      1h incubation at RT in 5% NFDM/TBST.

    • ELISA - Anti-Avian Influenza A Neuraminidase antibody (ab21305)
      ELISA - Anti-Avian Influenza A Neuraminidase antibody (ab21305)

      Validation with Avian Influenza NA Protein Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies: ab21305, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1/10000 dilution, incubated at RT for 1 hr.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (3)

    发表研究结果有使用 ab21305？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab21305 被引用在 3 文献中.

    • Wu CY  et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PLoS One 7:e42363 (2012). PubMed: 22879951
    • Wu CY  et al. Mammalian expression of virus-like particles for advanced mimicry of authentic influenza virus. PLoS One 5:e9784 (2010). WB, ICC/IF . PubMed: 20339535
    • Su CY  et al. In vitro evaluation of neuraminidase inhibitors using the neuraminidase-dependent release assay of hemagglutinin-pseudotyped viruses. Antiviral Res 79:199-205 (2008). PubMed: 18453004

    客户评价及客户问答

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    Question

    After contacting with this customer, he provided the information as follows, He wants to know that ab21304 might recognize the strain A/WSN/1933(H1N1) virus ;NCBI GI 194352376 or not. http://www.ncbi.nlm.nih.gov/protein/194352376 1 mnpnqkiiti gsicmvvgii slilqignii siwishsiqt gnqnhtgicn qgsitykvva 61 gqdstsvilt gnsslcpirg waihskdngi rigskgdvfv irepfiscsh lecrtffltq 121 gallndkhsr gtfkdrspyr almscpvgea pspynsrfes vawsasachd gmgwltigis 181 gpddgavavl kyngiiteti kswrknilrt qesectcvng scftimtdgp sdglasykif 241 kiekgkvtks ielnapnshy eecscypdtg kvmcvcrdnw hgsnrpwvsf dqnldykigy 301 icsgvfgdnp rpkdgtgscg pvsadgangv kgfsykygng vwigrtksds srhgfemiwd 361 pngwtetdsr fsmrqdvvam tdrsgysgsf vqhpeltgld cmrpcfwvel irglpeenai 421 wtsgsiisfc gvnsdtvdws wpdgaelpft idk Could you please help this customer to check the alignment for him, because he will decide whether he buy or not. thank you very much！ Sincerely yours,

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    Answer

    Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase antibody: 87% We recommend alignment shoudl be over 85% to predict that an antibody will detect. Therefore, there is likely to be some crossreactivity with ab21304, but ab21305 may be a better choice based on the alignment scores. Please check the online datasheet to ensure this has been tested in the application the customer would like to use. Click here (or use the following: https://www.abcam.com/Avian-Influenza-A-Neuraminidase-antibody-ab21305.html). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    Dear Sir/Madam My name is Shenshen Cai, currently working at University of Pennsylvania on some antigenic peptide projects. I see some H5N1 antibodies in your catalog. May I know the sequences of the immunogenic peptides you used to raise your antibodies ab21292, ab21305, ab21297, ab21304? thank you very much, I look forward to hearing from you.

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    Answer

    Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Could you please let us know whether this antibody can detect the NA protein of H5N1. We are looking forward to your replying. Thank you very much. Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. This product should detect the protein. Unfortunately, we have only been able to test the antibody against the immunogen peptide in ELISA.

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