Anti-Avian甲型流感Hemagglutinin抗体(ab62490)
Key features and details
- Goat polyclonal to Avian Influenza A Hemagglutinin
- Suitable for: WB, ELISA
- Isotype: IgG
概述
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产品名称
Anti-Avian甲型流感Hemagglutinin抗体
参阅全部 Avian Influenza A Hemagglutinin 一抗 -
描述
山羊多克隆抗体to Avian甲型流感Hemagglutinin -
宿主
Goat -
特异性
This antibody reacts with the Hemagglutinin 4 protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.
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经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: H5N1 subtype -
免疫原
A synthetic peptide corresponding to 10 amino acids near the center of the Hemagglutinin 4 protein (Avian Influenza A).
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阳性对照
- WB: H5N1 Hemagglutinin recombinant protein. ELISA: H5N1 Hemagglutinin recombinant protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62490于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 64 kDa).
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| ELISA |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 64 kDa). |
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ELISA
Use a concentration of 1 µg/ml. |
靶标
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相关性
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability. -
细胞定位
Cell Membrane -
别名
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
see all
图片
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Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62490)Lane 1 : Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 0.5 µg/ml
Lane 2 : Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 1 µg/ml
All lanes : 30 ng of H5N1 Hemagglutinin recombinant protein
Secondary
All lanes : Rabbit anti-goat IgG HRP conjugate at 1/10000 dilution
Predicted band size: 64 kDa1h incubation at RT in 5% NFDM/TBST.
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ELISA - Anti-Avian Influenza A Hemagglutinin antibody (ab62490)Validation with H5N1 HA Protein Coating Antigen: H5N1 Hemagglutinin recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies: ab62490, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Rabbit anti-goat HRP at 1/10,000 dilution, incubated at RT for 1 hr.
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Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62490)All lanes : Anti-Avian Influenza A Hemagglutinin antibody (ab62490) at 1 µg/ml
Lane 1 : 5ng Recombinant Hemagglutinin
Lane 2 : 25ng Recombinant Hemagglutinin
Predicted band size: 64 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62490 尚未被引用在任何文献中。