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重组RabMAb

重组Anti-AVEN抗体[EPR4720] (ab133285)

Reviews (1) Submit a question References (1)
Western blot - Anti-AVEN antibody [EPR4720] (ab133285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
  • Immunocytochemistry/ Immunofluorescence - Anti-AVEN antibody [EPR4720] (ab133285)
  • Flow Cytometry - Anti-AVEN antibody [EPR4720] (ab133285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
  • Anti-AVEN antibody [EPR4720] (ab133285)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4720] to AVEN
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-AVEN抗体[EPR4720]
    参阅全部 AVEN 一抗

  • 描述

    兔单克隆抗体[EPR4720] to AVEN

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human, Mouse and Rat testis tissue; ICC/IF: Ramos cells; Flow Cyt: Ramos cells. WB: Ramos, HeLa, RAW 264.7 and PC-12 whole cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133285于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/40.
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50- 1/100.
ICC/IF
1/50 - 1/250.
说明
Flow Cyt
1/40.
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50- 1/100.
ICC/IF
1/50 - 1/250.

靶标

图片

  • Western blot - Anti-AVEN antibody [EPR4720] (ab133285)
    Western blot - Anti-AVEN antibody [EPR4720] (ab133285)
    All lanes : Anti-AVEN antibody [EPR4720] (ab133285) at 1/1000 dilution (Purified)

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunocytochemistry/ Immunofluorescence - Anti-AVEN antibody [EPR4720] (ab133285)
    Immunocytochemistry/ Immunofluorescence - Anti-AVEN antibody [EPR4720] (ab133285)

    Immunocytochemistry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling AVEN with purified ab133285 at 1/50 dilution (8.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-AVEN antibody [EPR4720] (ab133285)
    Flow Cytometry - Anti-AVEN antibody [EPR4720] (ab133285)
    Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labelling AVEN with purified ab133285 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AVEN antibody [EPR4720] (ab133285)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Anti-AVEN antibody [EPR4720] (ab133285)
    Anti-AVEN antibody [EPR4720] (ab133285)

文献 (1)

发表研究结果有使用 ab133285？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab133285 被引用在 1 文献中.

  • O'Doherty AM  et al. Expression of granulosa cell microRNAs, AVEN and ATRX are associated with human blastocyst development. Mol Reprod Dev N/A:N/A (2018). PubMed: 29693772

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-AVEN antibody [EPR4720]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Chicken Tissue sections (Embryonic chick brainstem)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Tween-20 in TBS
Specification
Embryonic chick brainstem
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Forrest Weghorst

Verified customer

提交于 Dec 07 2017

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