AB224035

Anti-AUTS2抗体

Name: AUTS2抗体 (ab224035)| Abcam中文官网
Brand: Abcam Limited
SKU: AB224035
Price: 5757 CNY
Availability: InStock

Anti-AUTS2 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal AUTS2 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AUTS2 aa 1000-1150.

查看别名

KIAA0442, AUTS2, Autism susceptibility gene 2 protein

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling AUTS2 with ab224035 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemical analysis of human prostate tissue labeling AUTS2 in the nucleus of glandular cells with ab224035 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AUTS2 antibody (AB224035)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AUTS2 antibody (AB224035)

Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling AUTS2 in the nucleus of neuronal cells with ab224035 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemical analysis of human skin tissue labeling AUTS2 in the nucleus with ab224035 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemical analysis of human skeletal muscle tissue labeling AUTS2 with ab224035 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemical analysis of human cerebral cortex tissue labeling AUTS2 in the nucleus of neuronal cells with ab224035 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemical analysis of mouse embryo (E14) cortical plate tissue labeling AUTS2 in the nucleus of neuronal cells with ab224035 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human AUTS2 aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information.

Q8WXX7

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AUTS2 also known as the Autism Susceptibility Gene 2 is a protein encoded by the AUTS2 gene and has a mass of approximately 141 kDa. This protein is expressed in several regions of the brain including the cerebral cortex hippocampus and cerebellum. It plays a role in neuronal development where it impacts neural progenitor cell proliferation and differentiation. Expression of AUTS2 is often regulated during key neurodevelopmental stages.

Biological function summary

The AUTS2 protein is involved in chromatin remodeling impacting gene expression regulation during brain development. It functions as part of a complex involving other transcription factors and regulators to influence diverse neural processes. This involvement in chromatin remodeling highlights its role in controlling when and how genes are activated within cells impacting neurodevelopmental processes significantly.

Pathways

AUTS2 participates in the regulation of neuronal signaling pathways such as the Wnt signaling pathway which is key for cell fate determination during development. Proteins like TCF/LEF are related to AUTS2 through the Wnt signaling pathway where they serve as transcriptional mediators affected by the pathway's activity. This connection indicates AUTS2's role in maintaining the balance between progenitor cell proliferation and differentiation in neural tissues.

AUTS2 has a significant association with autism spectrum disorders (ASD) and intellectual disability. Disruptions or mutations in the AUTS2 gene often associate with these neurodevelopmental conditions. The protein also has connections with other proteins like CHD8 which is often mutated in autism suggesting that alterations affecting AUTS2 could influence broader functional networks in the brain responsible for cognitive development and behavior.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 25519132). The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B (PubMed : 25519132). As a consequence, the complex mediates transcriptional activation (PubMed : 25519132). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (By similarity).

See full target information AUTS2

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 19:43 PubMed35135580

2022

PD-L1 signaling in reactive astrocytes counteracts neuroinflammation and ameliorates neuronal damage after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Gao,Wei Li,Fahim Syed,Fang Yuan,Ping Li,Qigui Yu

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AUTS2抗体

Anti-AUTS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunocytochemistry/ Immunofluorescence - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody (AB224035)