重组Anti-Aurora B抗体[EPR25417-73] - BSA and Azide free
Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free
- BOND RX™ Validated
- Advanced Validation
- RabMAb
- Recombinant
- 了解详情
Be the first to review this product! Submit a review
|
(0 Publication)
Rabbit Recombinant Monoclonal Aurora B antibody. Carrier free. Suitable for mIHC, ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant full length protein - Mouse samples.
查看别名
Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5, Aurkb, Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, ARK-2, Aurora-related kinase 2
- mIHC
Lab
Multiplex immunohistochemistry - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Aurora B with ab287960 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were co-stained with DRAQ5 to differentiate cell cycle phase.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse colon. The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Aurora B with ab287960 at 1/50 (10.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in NIH/3T3 cells in M phase is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse testis. The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse embryo 12.5 dpc tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse embryo 12.5 dpc . The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Negative control : no staining on mouse lung. The section was incubated with ab287960 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L-929 cells labelling Aurora B with ab287960 at 1/50 (10.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in L-929 cells in M phase is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Aurora B was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with anti aurora b antibody epr2541773 immunoprecipitation nih3t3.jpg at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using anti aurora b antibody epr2541773 immunoprecipitation NIH/3T3 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2 : ab287960 IP in NIH/3T3 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab287960 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes
This blot was developed using a higher sensitivity ECL substrate.
All lanes:
Immunoprecipitation - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>)
Predicted band size: 39 kDa
false
- WB
Lab
Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 3 minutes
All lanes:
Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution
Lane 1:
L-929 ( mouse connective tissue fibroblast) whole cell lysate at 20 µg
Lane 2:
L-929 treated with 100ng/ml nocodazole for 20 hours whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 39 kDa
false
- WB
Lab
Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Exposure time : 3 minutes
All lanes:
Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution
All lanes:
C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 39 kDa
false
- WB
Lab
Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
This data was developed using ab287960, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 5.5 seconds
All lanes:
Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution
Lane 1:
His-tagged mouse Aurora B recombinant protein (aa13-261) 10ng
Lane 2:
His-tagged mouse Aurora C recombinant protein (aa5-237) 10ng
Lane 3:
GST-tagged mouse Aurora A recombinant protein (fl-length) 10ng
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 39 kDa
false
不同偶联物与剂型 (1)
-
Anti-Aurora B antibody [EPR25417-73]
反应性数据
产品详情
ab287965 is the carrier-free version of ab287960.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Aurora B kinase functions to ensure proper chromosome alignment segregation and cytokinesis during mitosis and meiosis. It phosphorylates various substrates to facilitate processes like correction of kinetochore-microtubule attachments and regulation of the mitotic checkpoint. As a part of the CPC Aurora B acts in coordination with other proteins to control these cellular events ensuring that cells divide accurately and safely.
Pathways
Aurora B integrates into the cell cycle and mitotic pathways operating closely with the spindle assembly checkpoint. It interacts with proteins like CENP-A and CENP-E ensuring error-free chromosome segregation. Aurora B's activity within these pathways is critical for maintaining genomic stability and preventing aneuploidy a condition linked to improper chromosome number.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com