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AB287965

重组Anti-Aurora B抗体[EPR25417-73] - BSA and Azide free

Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free

  • BOND RX™ Validated
  • Advanced Validation
  • RabMAb
  • Recombinant
  • 了解详情

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Rabbit Recombinant Monoclonal Aurora B antibody. Carrier free. Suitable for mIHC, ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant full length protein - Mouse samples.

查看别名

Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5, Aurkb, Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, ARK-2, Aurora-related kinase 2

12 Images
Multiplex immunohistochemistry - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
Flow Cytometry (Intracellular) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Aurora B with ab287960 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were co-stained with DRAQ5 to differentiate cell cycle phase.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse colon. The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Aurora B with ab287960 at 1/50 (10.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in NIH/3T3 cells in M phase is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse testis. The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse embryo 12.5 dpc tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Nuclear staining on mouse embryo 12.5 dpc . The section was incubated with ab287960 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling Aurora B with ab287960 at 1/300 (1.803 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Negative control : no staining on mouse lung. The section was incubated with ab287960 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L-929 cells labelling Aurora B with ab287960 at 1/50 (10.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in L-929 cells in M phase is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Immunoprecipitation - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • IP

Supplier Data

Immunoprecipitation - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Aurora B was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with anti aurora b antibody epr2541773 immunoprecipitation nih3t3.jpg at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using anti aurora b antibody epr2541773 immunoprecipitation NIH/3T3 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

Lane 2 : ab287960 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab287960 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>)

Predicted band size: 39 kDa

false

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • WB

Lab

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution

Lane 1:

L-929 ( mouse connective tissue fibroblast) whole cell lysate at 20 µg

Lane 2:

L-929 treated with 100ng/ml nocodazole for 20 hours whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • WB

Lab

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)
  • WB

Lab

Western blot - Anti-Aurora B antibody [EPR25417-73] - BSA and Azide free (AB287965)

This data was developed using ab287960, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-Aurora B antibody [EPR25417-73] (<a href='/products/primary-antibodies/aurora-b-antibody-epr25417-73-ab287960'>ab287960</a>) at 1/1000 dilution

Lane 1:

His-tagged mouse Aurora B recombinant protein (aa13-261) 10ng

Lane 2:

His-tagged mouse Aurora C recombinant protein (aa5-237) 10ng

Lane 3:

GST-tagged mouse Aurora A recombinant protein (fl-length) 10ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25417-73

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat

应用

WB, Flow Cyt (Intra), IHC-P, ICC/IF, mIHC, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Rat species is recommended based on WB result only.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Mouse": { "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

ab287965 is the carrier-free version of ab287960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aurora B also known as Aurora kinase B or AURKB is a serine/threonine protein kinase with a molecular mass of approximately 39 kDa. This protein expresses across various cell types and tissues playing a critical role during cell division. It localizes to the centromeres during early mitosis and later associates with the central spindle and midbody. Aurora B is a component of the chromosomal passenger complex (CPC) which includes other proteins such as INCENP survivin and borealin essential for its function.
Biological function summary

Aurora B kinase functions to ensure proper chromosome alignment segregation and cytokinesis during mitosis and meiosis. It phosphorylates various substrates to facilitate processes like correction of kinetochore-microtubule attachments and regulation of the mitotic checkpoint. As a part of the CPC Aurora B acts in coordination with other proteins to control these cellular events ensuring that cells divide accurately and safely.

Pathways

Aurora B integrates into the cell cycle and mitotic pathways operating closely with the spindle assembly checkpoint. It interacts with proteins like CENP-A and CENP-E ensuring error-free chromosome segregation. Aurora B's activity within these pathways is critical for maintaining genomic stability and preventing aneuploidy a condition linked to improper chromosome number.

Aurora B's malfunction associates with cancer and chromosomal instability. Overexpression or dysregulation of Aurora B kinase frequently occurs in several types of cancer contributing to tumorigenesis by promoting abnormal cell division. Proteins such as p53 a well-known tumor suppressor often become functionally compromised when Aurora B activity is altered further advancing disease progression. Aurora B also holds implications in other disorders marked by faulty cell cycle regulation emphasizing its potential as a therapeutic target.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis (By similarity). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly (By similarity). Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis (By similarity). Required for central/midzone spindle assembly and cleavage furrow formation (By similarity). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage : phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (By similarity). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP (By similarity). Phosphorylation of INCENP leads to increased AURKB activity (By similarity). Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3 (By similarity). A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres (By similarity). Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively) (PubMed : 11784863). AURKB is also required for kinetochore localization of BUB1 and SGO1 (By similarity). Phosphorylation of p53/TP53 negatively regulates its transcriptional activity (By similarity). Key regulator of active promoters in resting B- and T-lymphocytes : acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes (PubMed : 24034696). Acts as an inhibitor of CGAS during mitosis : catalyzes phosphorylation of the N-terminus of CGAS during the G2-M transition, blocking CGAS liquid phase separation and activation, and thereby preventing CGAS-induced autoimmunity (By similarity). Phosphorylates KRT5 during anaphase and telophase (PubMed : 29518391). Phosphorylates ATXN10 which promotes phosphorylation of ATXN10 by PLK1 and may play a role in the regulation of cytokinesis and stimulating the proteasomal degradation of ATXN10 (By similarity).
See full target information Aurkb

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