Anti-Aurora A (phospho T288)抗体(ab83968)
Key features and details
- Rabbit polyclonal to Aurora A (phospho T288)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Aurora A (phospho T288)抗体
参阅全部 Aurora A 一抗 -
描述
兔多克隆抗体to Aurora A (phospho T288) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Aurora A (phospho T288).
Database link: NP_003591.2 -
阳性对照
- NBF fixed asynchronous HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to Phospho Aurora A immobilized on a solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/100 - 1/500.
Formaldehyde fixed cells. Permeabilisation with Triton-X100 is recommended. |
| 说明 |
|---|
|
ICC/IF
1/100 - 1/500. Formaldehyde fixed cells. Permeabilisation with Triton-X100 is recommended. |
靶标
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功能
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. -
组织特异性
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
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别名
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora A (phospho T288) antibody (ab83968)ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100. -
Immunocytochemistry/ Immunofluorescence - Anti-Aurora A (phospho T288) antibody (ab83968)ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
Mock phosphatase treated section (left), or calf intestinal phosphatase treated section (right).
Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab83968 被引用在 6 文献中.
- Chiu SC et al. The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP. Cell Commun Signal 21:156 (2023). PubMed: 37370099
- Biswas S et al. PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay. Heliyon 8:e11656 (2022). PubMed: 36458309
- Kuriyama R & Fisher CR A novel mitosis-specific Cep215 domain interacts with Cep192 and phosphorylated Aurora A for organization of spindle poles. J Cell Sci 133:N/A (2020). PubMed: 33376154
- Kiseleva AA et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs. Clin Cancer Res 25:4179-4193 (2019). PubMed: 30867219
- Blas-Rus N et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun 7:11389 (2016). PubMed: 27091106
- Whalley HJ et al. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun 6:7437 (2015). ICC/IF ; Human . PubMed: 26078008