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Cell Biology Cell Cycle Cell Division Spindle

Anti-Aurora A抗体- Centrosome Marker (ab1287)

  • Datasheet
  • SDS
Reviews (1)Q&A (9)References (34)

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Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (ab1287)

    Key features and details

    • Rabbit polyclonal to Aurora A - Centrosome Marker
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Aurora A抗体- Centrosome Marker
      参阅全部 Aurora A 一抗
    • 描述

      兔多克隆抗体to Aurora A - Centrosome Marker
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IPmore details
    • 种属反应性

      与反应: Human
      预测可用于: Chimpanzee
    • 免疫原

      Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465)

    • 阳性对照

      • IP: HeLa
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Spindle
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Aurora
      • Cancer
      • Cell cycle
      • Cell division
      • Cancer
      • Tumor biomarkers
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Aurora A protein (ab42595)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1287于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IP
    Use a concentration of 0.1 µg/ml.
    说明
    IP
    Use a concentration of 0.1 µg/ml.

    靶标

    • 功能

      Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
    • 组织特异性

      Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
    • 序列相似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰

      Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
    • 细胞定位

      Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
    • Target information above from: UniProt accession O14965 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 6790 Human
      • Omim: 603072 Human
      • SwissProt: O14965 Human
      • Unigene: 250822 Human
      • 别名

        • AIK antibody
        • ARK-1 antibody
        • ARK1 antibody
        • AURA antibody
        • Aurka antibody
        • Aurora 2 antibody
        • Aurora A antibody
        • Aurora kinase A antibody
        • Aurora-related kinase 1 antibody
        • Aurora/IPL1 like kinase antibody
        • AURORA/IPL1-like kinase antibody
        • Aurora/IPL1-related kinase 1 antibody
        • AURORA2 antibody
        • Breast tumor-amplified kinase antibody
        • BTAK antibody
        • hARK1 antibody
        • IAK antibody
        • IPL1 related kinase antibody
        • MGC34538 antibody
        • OTTHUMP00000031340 antibody
        • OTTHUMP00000031341 antibody
        • OTTHUMP00000031342 antibody
        • OTTHUMP00000031343 antibody
        • OTTHUMP00000031344 antibody
        • OTTHUMP00000031345 antibody
        • OTTHUMP00000166071 antibody
        • OTTHUMP00000166072 antibody
        • PPP1R47 antibody
        • Protein phosphatase 1, regulatory subunit 47 antibody
        • Serine/threonine kinase 15 antibody
        • Serine/threonine kinase 6 antibody
        • Serine/threonine protein kinase 15 antibody
        • Serine/threonine-protein kinase 15 antibody
        • Serine/threonine-protein kinase 6 antibody
        • Serine/threonine-protein kinase aurora-A antibody
        • STK15 antibody
        • STK6 antibody
        • STK6_HUMAN antibody
        • STK7 antibody
        see all

      图片

      • Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (ab1287)
        Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (ab1287)

        Immunoprecipitation of Human Aurora A with ab1287 at 0.1 µg/mL.

        Lane 1: ab1287 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

        Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

        Detection: Chemiluminescence

      实验方案

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (34)

      发表研究结果有使用 ab1287?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1287 被引用在 34 文献中.

      • Wang R  et al. Selective targeting of non-centrosomal AURKA functions through use of a targeted protein degradation tool. Commun Biol 4:640 (2021). PubMed: 34050235
      • Yasumizu Y  et al. MUC1-C regulates lineage plasticity driving progression to neuroendocrine prostate cancer. Nat Commun 11:338 (2020). PubMed: 31953400
      • Capalbo L  et al. The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nat Commun 10:4513 (2019). PubMed: 31586073
      • Yang C  et al. Effects of AURKA-mediated degradation of SOD2 on mitochondrial dysfunction and cartilage homeostasis in osteoarthritis. J Cell Physiol N/A:N/A (2019). PubMed: 30811038
      • Willems E  et al. Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration. Oncogene 38:73-87 (2019). PubMed: 30082913
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 10 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Aurora A antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Michael Porter

      Verified customer

      提交于 Aug 14 2006

      Question

      I am doing IHC-P and WB; IHC-P is of mouse brain tumor of injected human tumor cells; WB of tumor cell lysate shows expected band size; IHC shows no staining; IHC used primary at 1:250; secondary is HRP conjugated.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 06 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      回复于 Jul 06 2012

      Question

      I am looking at the Anti-Aurora A antibody (ab1287) for immunohistochemistry, and I wonder what you recommend we use as a positive control?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 11 2014

      Answer

      In general, we recommend the tissue you see on the datasheet, in this case a pancreatic tumor. We offer sections of pancreatic adenocarcinoma, not of the exact same tumor, but we assume the staining will be similar. Here is a link to the datasheet for the sections.

      https://www.abcam.com/pancreas-tumor-human-adenocarcinoma-tissue-slides-ab5036.html

      I also recommend consulting the Human Protein Atlas. The entry for Aurora A is at these links:

      http://www.proteinatlas.org/ENSG00000087586/tissue

      http://www.proteinatlas.org/ENSG00000087586/tissue/staining+overview

      The current data is from only two antibodies, though.

      For normal tissue, the UniProt entry for human Aurora A (http://www.uniprot.org/uniprot/O14965) has this note, but without a reference.

      Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

      We have sections of many paraffin-embedded tissues, including testis, which are easy to find by typing the name of the tissue in the search box on our website and filtering the list of results by product type.

      Read More

      Tom Ruyle

      Abcam Scientific Support

      回复于 Feb 11 2014

      Question

      Dear technical support team:



      This customer has highly interested in ab1287 (Anti-Aurora A antibody), he would like to know the currently in stock lot number of this antibody. Would you please help this customer to solve the problem?



      Thanks for your kindly assistance.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 11 2012

      Answer

      Thank you for your enquiry and your interest in our products.

      I can confirm that we have 9 vials of batch: GR4766-7 in our UK stock.

      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      回复于 May 11 2012

      Question

      BATCH NUMBER 165680 ORDER NUMBER 164559 DESCRIPTION OF THE PROBLEM enquiry ref: 889878 This package was simple, only used one small ice bag ANTIBODY STORAGE CONDITIONS The Abs were in RT when we received ADDITIONAL NOTES As we haven't positive control slide for the Aurora A, and we are very busy, we didn't do IHC, but we did Western Blotting for all these four abs that we ordered and you shipped together, we don't know if this Aurora A is working or not, but the ER-a is not working, so we ask replace Aurora A too. Attached is Western Blotting results by JPE format, if you can't open please let me know Following are my more detail standpoints. The Abs were definitely in RT when we received This package was simple, only used one small ice bag (around half pond). If you still persist in, your technique department can simulate this shipment, see what will go on. We paid shipping fee, inside are thousand dollar worth four Abs, abcam should be responsible for the Abs¡¯ quality using correct ice package.This was two days and hot summer shipment, we don¡¯t know what UPS ¡® Delivery status was, especially on rigorous delivery truck.Abs¡¯ properties are very as you know, even unhappy with us when received The four Abs, we still set a Western Blotting test at 2nd day: Cyclin b1 and PCNA birth have correct bands, ER-a had not any band and we haven¡¯ IHC positive control slide for Aurora AWe bought in bulk mount of Abs from abcam or others every year, we have lots of experiments every week, we are very busy, hopefully, this is last enquiry on this case. Your understanding is my pleasure!

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2006

      Answer

      Thank you for your enquiry. I am afraid your image did not come through. All of our antibodies are stable at room temperature for at least 2 weeks. From the information you have given, I cannot tell whether there may be something in your protocol that can be optimized for better results, or if the antibody is really at fault. If you could fill out the form again with the details of your Western blot protocol, I can look into this further. Please find the link again below for your convenience. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=1287&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Aug 29 2006

      Question

      Customer would like to know for using ab1287 and ab2254 in IHC-formalin fixed paraffin embedded sections, at what pH for antigen retrieval?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 30 2004

      Answer

      Thank you for your enquiry. For ab1287, the originator has recommended using 0.1 M citrate buffer, pH 6.6 and 1mM EDTA. For ab2254, we would suggest microwave pre-treatment in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Jan 03 2005

      Question

      I need to know in detail where the antibody binds to aurora. If you could try to investigate this matter and give me an answer. I would be grateful.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 18 2004

      Answer

      I'm afraid the precise immunogen sequence used is proprietary. The binding site is between residues 50 and 100 of human Aurora A using the numbering given in entry NP_003591.1. I hope this information helps,

      Read More

      Abcam Scientific Support

      回复于 Nov 19 2004

      Question

      I have a question about the Aorora A antibody that I have ordered from You, from which terminus on the aurora A protein does it bind? If you know something about this I would be glad to know. Thank You..

      Read More

      Abcam community

      Verified customer

      Asked on Oct 22 2004

      Answer

      Thank you for your email. The immunogen used was a synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465). If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Oct 25 2004

      Question

      A customer would like more details with regard to storage buffer (esp. molarity) for ab1287.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 08 2004

      Answer

      Thank you for your enquiry. The buffer is 50 mM Tris, 5 mM Citrate and 40 mM Phosphate. If you need any further assistance please do not hesitate to get back in touch.

      Read More

      Abcam Scientific Support

      回复于 Sep 09 2004

      Question

      BATCH NUMBER 62608 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No nuclear staining seen, only cytoplasmic staining. SAMPLE Tissue, PRIMARY ANTIBODY Abcam ab1287, 1/250 in NSS/Tris buffered saline, 40mins, rtemp, wash for 5 mins. SECONDARY ANTIBODY Dako Duet Kit-K0492, as advised in kit DETECTION METHOD Dako Duet Kit-K0492, as advised in kit POSITIVE AND NEGATIVE CONTROLS USED Trying to get staining on breast and colon tumour. ANTIBODY STORAGE CONDITIONS As advised. FIXATION OF SAMPLE Neutral buffered formalin ANTIGEN RETRIEVAL Heat mediated, using pressure cooker and microwave techniques (separately) BLOCKING CONDITIONS Normal swine serum

      Read More

      Abcam community

      Verified customer

      Asked on Sep 03 2004

      Answer

      Thank you for your enquiry and for sending us your detailed protocol. We would suggest blocking the non-specific binding sites with 1%BSA for 1 hr and incubating the samples with the primary antibody for 2 hrs (40 min is too short). This antibody ahs been tested for IHC using human pancreatic tumor sections, so it may be worth using this type of tissue sections as positive control. Should you still have problem with this antibody, please do not hesitate to contact us again.

      Read More

      Abcam Scientific Support

      回复于 Sep 08 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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