Anti-Aurora A抗体- Centrosome Marker (ab1287)

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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I wish you the best of luck with your research.

In general, we recommend the tissue you see on the datasheet, in this case a pancreatic tumor. We offer sections of pancreatic adenocarcinoma, not of the exact same tumor, but we assume the staining will be similar. Here is a link to the datasheet for the sections.

https://www.abcam.com/pancreas-tumor-human-adenocarcinoma-tissue-slides-ab5036.html

I also recommend consulting the Human Protein Atlas. The entry for Aurora A is at these links:

http://www.proteinatlas.org/ENSG00000087586/tissue

http://www.proteinatlas.org/ENSG00000087586/tissue/staining+overview

The current data is from only two antibodies, though.

For normal tissue, the UniProt entry for human Aurora A (http://www.uniprot.org/uniprot/O14965) has this note, but without a reference.

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

We have sections of many paraffin-embedded tissues, including testis, which are easy to find by typing the name of the tissue in the search box on our website and filtering the list of results by product type.

Thank you for your enquiry and your interest in our products.

I can confirm that we have 9 vials of batch: GR4766-7 in our UK stock.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry. I am afraid your image did not come through. All of our antibodies are stable at room temperature for at least 2 weeks. From the information you have given, I cannot tell whether there may be something in your protocol that can be optimized for better results, or if the antibody is really at fault. If you could fill out the form again with the details of your Western blot protocol, I can look into this further. Please find the link again below for your convenience. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=1287&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. For ab1287, the originator has recommended using 0.1 M citrate buffer, pH 6.6 and 1mM EDTA. For ab2254, we would suggest microwave pre-treatment in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). If you have any additional questions, please contact us again.

I'm afraid the precise immunogen sequence used is proprietary. The binding site is between residues 50 and 100 of human Aurora A using the numbering given in entry NP_003591.1. I hope this information helps,

Thank you for your email. The immunogen used was a synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465). If you have any more questions, please contact us again.

Thank you for your enquiry. The buffer is 50 mM Tris, 5 mM Citrate and 40 mM Phosphate. If you need any further assistance please do not hesitate to get back in touch.

Thank you for your enquiry and for sending us your detailed protocol. We would suggest blocking the non-specific binding sites with 1%BSA for 1 hr and incubating the samples with the primary antibody for 2 hrs (40 min is too short). This antibody ahs been tested for IHC using human pancreatic tumor sections, so it may be worth using this type of tissue sections as positive control. Should you still have problem with this antibody, please do not hesitate to contact us again.