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重组RabMAb

重组Anti-ATTY抗体[EPR6121] (ab125000)

Submit a review Submit a question References (1)
Western blot - Anti-ATTY antibody [EPR6121] (ab125000)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] (ab125000)
  • OI-RD Scanning - Anti-ATTY antibody [EPR6121] (ab125000)
  • Anti-ATTY antibody [EPR6121] (ab125000)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6121] to ATTY
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ATTY抗体[EPR6121]
    参阅全部 ATTY 一抗

  • 描述

    兔单克隆抗体[EPR6121] to ATTY

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human ATTY aa 300-400. The exact sequence is proprietary.

  • 阳性对照

    • HeLa whole cell lysate (ab29545) can be used as a positive control in WB. HepG2 (treated with insulin), HepG2, and A549 cell lysates; Human brain tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125000于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.

  • 通路

    Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.

  • 疾病相关

    Defects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.

  • 序列相似性

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ATTY_HUMAN antibody
    • L-tyrosine:2-oxoglutarate aminotransferase antibody
    • TAT antibody
    • Tyrosine aminotransferase antibody
    • Tyrosine aminotransferase, cytosolic antibody
    see all

图片

  • Western blot - Anti-ATTY antibody [EPR6121] (ab125000)
    Western blot - Anti-ATTY antibody [EPR6121] (ab125000)
    All lanes : Anti-ATTY antibody [EPR6121] (ab125000) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate, treated with insulin
    Lane 2 : HepG2 cell lysate, untreated
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] (ab125000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] (ab125000)

    ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ATTY antibody [EPR6121] (ab125000)
    OI-RD Scanning - Anti-ATTY antibody [EPR6121] (ab125000)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ATTY antibody [EPR6121] (ab125000)
    Anti-ATTY antibody [EPR6121] (ab125000)

文献 (1)

发表研究结果有使用 ab125000？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab125000 被引用在 1 文献中.

  • Nandi SS  et al. Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes 65:3075-90 (2016). PubMed: 27411382

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