重组Anti-ATR (phospho S428)抗体[EPR2184] (ab178407)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)](https://www.abcam.cn/ps/products/178/ab178407/Images/ab178407-485099-AntiATR-phospho-S428-antibody-EPR2184-immunohistochemistry-thyroid-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2184] to ATR (phospho S428)
- Suitable for: Dot blot, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATR (phospho S428)抗体[EPR2184]
参阅全部 ATR 一抗 -
描述
兔单克隆抗体[EPR2184] to ATR (phospho S428) -
宿主
Rabbit -
特异性
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
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经测试应用
适用于: Dot blot, IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC: Human breast tissue, human thyroid tissue; WB: Untreated HeLa, HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate;
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2184 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178407于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 300 kDa.
|
| 说明 |
|---|
|
Dot blot
1/1000. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 300 kDa. |
靶标
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功能
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
组织特异性
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
疾病相关
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
序列相似性
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Phosphorylated; autophosphorylates in vitro. -
细胞定位
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
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数据库链接
- Entrez Gene: 545 Human
- Omim: 601215 Human
- SwissProt: Q13535 Human
- Unigene: 271791 Human
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别名
- Ataxia telangiectasia and Rad3 related antibody
- Ataxia telangiectasia and Rad3-related protein antibody
- ATR antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
Immunohistochemical analysis of Paraffin-embedded human thyroid tissue sections labelling ATR with ab178407 at 1/5000 dilution followed by ready to use secondary ab209101 Rabbit specific IHC polymer detection kit HRP/DAB. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Nuclear staining on human thyroid without alkaline phosphatase (or Lambda Protein Phosphatase) treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab178407 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)](https://www.abcam.cn/ps/products/178/ab178407/Images/ab178407-485098-AntiATR-phospho-S428-antibody-EPR2184-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)Immunohistochemical analysis of Paraffin-embedded human breast tissue sections labelling ATR with ab178407 at 1/5000 dilution followed by ready to use secondary ab209101 Rabbit specific IHC polymer detection kit HRP/DAB. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Nuclear staining on human breast without alkaline phosphatase (or Lambda Protein Phosphatase) treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab178407 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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![Western blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)](https://www.abcam.cn/ps/products/178/ab178407/Images/ab178407-485097-AntiATR-phospho-S428-antibody-EPR2184-western-blot-hela-human.jpg)
Western blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate
Lane 3 : HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 300 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 60 seconds
We are unsure of the nature of the 160kDa band.
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![Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)](https://www.abcam.cn/ps/products/178/ab178407/Images/ab178407-485095-AntiATR-phospho-S428-antibody-EPR2184-dot-blot.jpg)
Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)Dot blot analysis using 1/1000 dilution ab178407 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary at 1/100000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Lane 1: ATR non-phospho peptide
Lane 2: ATR S428 phospho peptide
Lane 3: ATR S435 phospho peptide
Lane 4: ATR S428+S435 phospho peptide
Exposure time: 3 minutes
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![Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)](https://www.abcam.cn/ps/products/178/ab178407/Images/ab178407-6-benefits-of-recombinant-antibodies.png)
Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab178407 被引用在 23 文献中.
- Zhong NS et al. HELQ suppresses migration and proliferation of non-small cell lung cancer cells by repairing DNA damage and inducing necrosis. Cell Biol Int 47:188-200 (2023). PubMed: 36183369
- Sundararajan V et al. Nuclear pCHK1 as a potential biomarker of increased sensitivity to ATR inhibition. J Pathol 259:194-204 (2023). PubMed: 36373784
- Wang Q et al. Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription. J Bone Miner Res 38:427-442 (2023). PubMed: 36625422
- Tao L et al. Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51. Nat Commun 14:7430 (2023). PubMed: 37973845
- Pádua JDB et al. Prognostic Value of the Immunohistochemical Expression of RAD51 and BRCA2 in Gastric Adenocarcinoma. J Histochem Cytochem 70:199-210 (2022). PubMed: 34978208