Anti-ATR抗体(ab2905)
Key features and details
- Rabbit polyclonal to ATR
- Suitable for: ChIP, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ATR抗体
参阅全部 ATR 一抗 -
描述
兔多克隆抗体to ATR -
宿主
Rabbit -
经测试应用
适用于: ChIP, WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Fusion protein, corresponding to amino acids 400-460 of Human ATR.
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阳性对照
- WB: K562 lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration. PubMed: 20647539
|
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| WB | (17) |
1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells).
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| IP | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ChIP
Use at an assay dependent concentration. PubMed: 20647539 |
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WB
1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells). |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
组织特异性
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
疾病相关
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
序列相似性
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Phosphorylated; autophosphorylates in vitro. -
细胞定位
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
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数据库链接
- Entrez Gene: 545 Human
- Entrez Gene: 245000 Mouse
- Omim: 601215 Human
- SwissProt: Q13535 Human
- SwissProt: Q9JKK8 Mouse
- Unigene: 271791 Human
- Unigene: 212462 Mouse
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别名
- Ataxia telangiectasia and Rad3 related antibody
- Ataxia telangiectasia and Rad3-related protein antibody
- ATR antibody
see all
图片
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Western blot - Anti-ATR antibody (ab2905)Western blot on ATR in K562 whole cell lysate using ab2905.
数据表及文件
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Datasheet download
文献 (38)
ab2905 被引用在 38 文献中.
- Wu H et al. miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis. J Cell Mol Med 25:1896-1910 (2021). PubMed: 33465281
- Chou HC et al. The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure. Elife 10:N/A (2021). PubMed: 33522487
- Zhang H et al. CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSa/ATM-p73 axis in bladder cancer. Mol Cancer 20:70 (2021). PubMed: 33874956
- Fan J et al. Oncolytic herpes simplex virus and temozolomide synergistically inhibit breast cancer cell tumorigenesis in vitro and in vivo. Oncol Lett 21:99 (2021). PubMed: 33376532
- Cheng A et al. ATM loss disrupts the autophagy-lysosomal pathway. Autophagy 17:1998-2010 (2021). PubMed: 32757690
