重组Anti-ATPB抗体[EPR11990] (ab170947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11990] to ATPB
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATPB抗体[EPR11990]
参阅全部 ATPB 一抗 -
描述
兔单克隆抗体[EPR11990] to ATPB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human ATPB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: P06576 -
阳性对照
- HepG2; 293T; HeLa and HT-29 cell lysates; Human heart and liver tissues; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11990 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170947于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100. |
|
IP |
1/10 - 1/100.
|
说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 56 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ICC/IF
1/500. For unpurified use at 1/50 - 1/100. |
IP
1/10 - 1/100. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 171374 Rat
- GenBank: BC016512 Human
- Omim: 102910 Human
- SwissProt: P06576 Human
- SwissProt: P56480 Mouse
- SwissProt: P10719 Rat
see all -
别名
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
see all
图片
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All lanes : Anti-ATPB antibody [EPR11990] (ab170947) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ATPB with purified ab170947 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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ab170947 showing +ve staining in Human normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab170947 showing +ve staining in Human normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATPB with ab170947 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ATPB with ab170947 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling ATPB using ab170947 at 1/50 dilution (green). DAPI nuclear staining (blue).
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Anti-ATPB antibody [EPR11990] (ab170947) at 1/10 dilution + HepG2 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab170947 被引用在 9 文献中.
- Tang Y et al. Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1-/- mice. J Neurochem 157:550-560 (2021). PubMed: 33305362
- Zhou H et al. Guhong Injection Protects Against Apoptosis in Cerebral Ischemia by Maintaining Cerebral Microvasculature and Mitochondrial Integrity Through the PI3K/AKT Pathway. Front Pharmacol 12:650983 (2021). PubMed: 34054531
- Liu Z et al. IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress. J Exp Clin Cancer Res 39:144 (2020). PubMed: 32727517
- Xiang Y et al. SR18292 exerts potent antitumor effects in multiple myeloma via inhibition of oxidative phosphorylation. Life Sci 256:117971 (2020). PubMed: 32553925
- Zhang J et al. Involvement of kindlin-2 in irisin's protection against ischaemia reperfusion-induced liver injury in high-fat diet-fed mice. J Cell Mol Med 24:13081-13092 (2020). PubMed: 32954626
- Fu H et al. Profiling of nuclear copper-binding proteins under hypoxic condition. Biometals 32:329-341 (2019). PubMed: 30739301
- Feng X et al. Feature Article: The involvement of mitochondrial fission in maintenance of the stemness of bone marrow mesenchymal stem cells. Exp Biol Med (Maywood) 244:64-72 (2019). PubMed: 30614257
- Speransky S et al. A novel RNA aptamer identifies plasma membrane ATP synthase beta subunit as an early marker and therapeutic target in aggressive cancer. Breast Cancer Res Treat 176:271-289 (2019). PubMed: 31006104
- Liu X et al. Copper levels affect targeting of hypoxia-inducible factor 1a to the promoters of hypoxia-regulated genes. J Biol Chem 293:14669-14677 (2018). PubMed: 30082314