Anti-ATPB抗体[4.3E8.D10] - Mitochondrial Marker (ab5432)

Thank you for your email. Yes, we do carry human mitochondrial lysates as well: Human Liver Mitochondrial lysate (ab110339) Human Heart Mitochondrial lysate (ab110337) However, both lysates come in WB loading buffer (ready to be used in WB) and not iso-osmotic buffer as the bovine lysate. Regarding the antibodies: ab5432: This antibody has not been tested with bovine samples yet, but the sequence homology between rat (immunogen) and bovine is 96%. If you would like to try the antibody in bovine, you can take advantage of our testing discount program. For details please see below. ab110280: This antibody has been tested with bovine samples in WB and would be guaranteed for this species and application. Please see lane 2 for the WB data with bovine mitochondria. *Testing discount program: Here is some information about how the testing discount program works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. *Abpromise guarantee: All our products are guaranteed for the tested species and applications as listed on the datasheet for 6 months after the purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Merci pour votre appel de l'autre jour.

Je viens de recevoir la réponse du laboratoire. Malheureusement l'épitope du ab5432 n'as pas été déterminé non plus à notre connaissance.

Je suis désolée de ne pas pouvoir vous mieux aider en cette occasion. Veuillez ne pas hésiter à nous recontacter si vous avez des autres questions.

Thank you for your enquiry and your patience. Based on the resources available for ATP Synthase Beta (ab5432), this product has not been epitope mapped yet. Occassionally customers will send us such information when they do the epitope mapping themselves, but I do not have any such information of this being done. Therefore, I would not know which domain was used as the immunogen. I apologize for not having this information.

Thank you for your enquiry. The epitopes have not been determined for these two monoclonal antibodies. Ab5432 and ab14730 are different clones, and ab5432 was made using intact rat mitochondria as the immunogen, whereas human whole heart mitochondria was used as the immunogen for ab14730. Ab5432 was tested for application in IP and IF, and ab14730 was tested for application in Western blot and Immunocytochemistry. Please contact us again if you have any additional questions.

Dr. Wang, Thank you for contacting us with your request. We do not have antibodies against all 13 of the proteins encoded by the mitochondrial genome yet. Right now we only seem to have an antibody against the COXI subunit, ab14705, which cross reacts with human, mouse, rat and cow. All the other antibodies we have against mitochondrial proteins are against ones encoded in the nucleus. I hope this is helpful to you, and keep checking with us as we add lots of new products each month and you may find what you need in the future.

Thank you for your enquiry. We are very sorry to hear you are having problem with this product. We regret to inform you that ab5432has not been tested for Western blot application only for IP and IF. Please could you take a look at the on-line datasheet. It cross-reacts with human, mouse and rat protein ATP Synthase beta but we do not have any information if it recognizes S.cereviciae. We would suggest using rat brain lysate as positive control along with the samples. If you need anything further or any help then please let us know.

We haven't tested this antibody in WB applications (ab5432 has been tested in Immunoprecipitation and Immunofluorescence), therefore we don't know. Although, by immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase from solubilized rat brain mitochondria.