Anti-ATP6V1C1抗体(ab272594)
Key features and details
- Rabbit polyclonal to ATP6V1C1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ATP6V1C1抗体 -
描述
兔多克隆抗体to ATP6V1C1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Orangutan
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免疫原
Recombinant fragment corresponding to Human ATP6V1C1 aa 300-400.
Database link: P21283 -
阳性对照
- IHC-P: Human stomach, cerebellum, cerebral cortex, and kidney tissues. WB: A431, NIH/3T3 and NBT-II cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the V-ATPase C subunit family. - Information by UniProt
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数据库链接
- Entrez Gene: 338089 Cow
- Entrez Gene: 528 Human
- Entrez Gene: 66335 Mouse
- Entrez Gene: 100171885 Orangutan
- Entrez Gene: 299971 Rat
- Omim: 603097 Human
- SwissProt: P21282 Cow
- SwissProt: P21283 Human
see all -
别名
- ATP6C antibody
- ATP6D antibody
- ATP6V1C1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1C1 antibody (ab272594)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ATP6V1C1 with ab272594 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1C1 antibody (ab272594)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ATP6V1C1 with ab272594 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1C1 antibody (ab272594)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling ATP6V1C1 with ab272594 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1C1 antibody (ab272594)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ATP6V1C1 with ab272594 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-ATP6V1C1 antibody (ab272594)All lanes : Anti-ATP6V1C1 antibody (ab272594) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat bladder carcinoma cell line) cell lysate -
Western blot - Anti-ATP6V1C1 antibody (ab272594)Anti-ATP6V1C1 antibody (ab272594) at 0.4 µg/ml + A431 (Human epidermoid carcinoma cell line) cell lysate
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272594 尚未被引用在任何文献中。