Anti-ATP6V1B2抗体(ab73404)
Key features and details
- Rabbit polyclonal to ATP6V1B2
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ATP6V1B2抗体
参阅全部 ATP6V1B2 一抗 -
描述
兔多克隆抗体to ATP6V1B2 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse ATP6V1B2.
参阅Abcam的专有抗源政策 (Peptide available as ab74898.) -
阳性对照
- This antibody gave a positive signal in the following tissue lysates: Rat hippocampus; Mouse hippocampus; Mouse kidney; Mouse testis; Human kidney; Human testis.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73404于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| IHC-P | (1) |
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
IP
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
细胞定位
Endomembrane system. Melanosome. Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 338082 Cow
- Entrez Gene: 526 Human
- Entrez Gene: 11966 Mouse
- Entrez Gene: 100173673 Orangutan
- Entrez Gene: 117596 Rat
- Omim: 606939 Human
- SwissProt: P31408 Cow
- SwissProt: P21281 Human
see all -
别名
- ATP6B1B2 antibody
- ATP6B2 antibody
- ATP6V1 B2 antibody
see all
图片
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Western blot - Anti-ATP6V1B2 antibody (ab73404)All lanes : Anti-ATP6V1B2 antibody (ab73404) at 1 µg/ml
Lane 1 : Hippocampus (Mouse) Tissue Lysate
Lanes 2 & 8 : Kidney (Mouse) Tissue Lysate
Lanes 3 & 9 : Testis (Mouse) Tissue Lysate
Lanes 4 & 10 : Human testis tissue lysate - total protein (ab30257)
Lanes 5 & 11 : Human kidney tissue lysate - total protein (ab30203)
Lanes 6 & 12 : Rat Hippocampus Tissue Lysate
Lane 7 : Hippocampus (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Lanes 1-6 were blocked with 5% BSA, Lanes 7-12 were blocked with 3% milk. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
- Anti-ATP6V1B2 antibody (ab73404)ATP6V1B2 was immunoprecipitated using 0.5mg Mouse Testis tissue lysate, 5µg of Rabbit polyclonal to ATP6V1B2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73404.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 57kDa; ATP6V1B2 -
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1B2 antibody (ab73404)ICC/IF image of ab73404 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73404, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B2 antibody (ab73404)IHC image of ATP6V1B2 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73404, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B2 antibody (ab73404)This image is courtesy of an anonymous Abreviewab73404 staining RAt incisor tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in 1% BSA/ 0.5% Triton X-100 in PBS) for 16 hours at 4°C. An undiluted peroxidase-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab73404 被引用在 22 文献中.
- Raas Q et al. Peroxisomal defects in microglial cells induce a disease-associated microglial signature. Front Mol Neurosci 16:1170313 (2023). PubMed: 37138705
- Sun J et al. Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy. Aging Cell 21:e13532 (2022). PubMed: 34905649
- Rafiq M et al. PLB-985 Neutrophil-Like Cells as a Model To Study Aspergillus fumigatus Pathogenesis. mSphere 7:e0094021 (2022). PubMed: 34986319
- Chen X et al. Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation. Nat Commun 13:7578 (2022). PubMed: 36481721
- Lu J et al. HPS6 Regulates the Biogenesis of Weibel-Palade Body in Endothelial Cells Through Trafficking v-ATPase to Its Limiting Membrane. Front Cell Dev Biol 9:743124 (2021). PubMed: 35252216