Anti-ATP6V1B2抗体(ab183887)
Key features and details
- Rabbit polyclonal to ATP6V1B2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ATP6V1B2抗体
参阅全部 ATP6V1B2 一抗 -
描述
兔多克隆抗体to ATP6V1B2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Recombinant fragment within Human ATP6V1B2 aa 37-320. The exact sequence is proprietary.
Database link: P21281 -
阳性对照
- U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysate/extract. Mouse and rat brain lysate/extract. Mouse kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/10000. Predicted molecular weight: 57 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 57 kDa. |
靶标
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功能
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
细胞定位
Endomembrane system. Melanosome. Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 338082 Cow
- Entrez Gene: 526 Human
- Entrez Gene: 11966 Mouse
- Entrez Gene: 117596 Rat
- Omim: 606939 Human
- SwissProt: P31408 Cow
- SwissProt: P21281 Human
- SwissProt: P62814 Mouse
see all -
别名
- ATP6B1B2 antibody
- ATP6B2 antibody
- ATP6V1 B2 antibody
see all
图片
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All lanes : Anti-ATP6V1B2 antibody (ab183887) at 1/1000 dilution
Lane 1 : U87-MG whole cell lysate
Lane 2 : SK-N-SH whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa7.5% SDS PAGE
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Immunohistochemical analysis of paraffin embedded mouse kidney labeling ATP6V1B2 with ab183887 at 1/500 dilution, showing cytosol staining.
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Anti-ATP6V1B2 antibody (ab183887) at 1/5000 dilution + Rat brain at 50 µg
Predicted band size: 57 kDa7.5% SDS PAGE
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Anti-ATP6V1B2 antibody (ab183887) at 1/5000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 57 kDa7.5% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab183887 被引用在 1 文献中.
- Kontaxi C et al. Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals. J Neurosci 43:2002-2020 (2023). PubMed: 36759195