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Anti-ATP5O抗体[4C11C10D12] (ab110276)

Reviews (1) Submit a question References (25)
Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)
  • Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)
  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

Key features and details

  • Mouse monoclonal [4C11C10D12] to ATP5O
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Cow, Human, African green monkey
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

概述

  • 产品名称

    Anti-ATP5O抗体[4C11C10D12]
    参阅全部 ATP5O 一抗

  • 描述

    小鼠单克隆抗体[4C11C10D12] to ATP5O

  • 宿主

    Mouse

  • 特异性

    Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Cow, Human, African green monkey
    不与反应: Mouse, Rat

  • 免疫原

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • 阳性对照

    • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells WB: Recombinant Human ATP5O protein (ab104549) cell lysate.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110276于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.

Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/500.
说明
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.

Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/500.

靶标

  • 功能

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

  • 序列相似性

    Belongs to the ATPase delta chain family.

  • 细胞定位

    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ATP synthase O subunit mitochondrial precursor antibody
      • ATP synthase subunit O antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
      • ATP5O antibody
      • ATPO antibody
      • ATPO_HUMAN antibody
      • mitochondrial antibody
      • Mitochondrial ATP synthase, O subunit antibody
      • Oligomycin sensitivity conferral protein antibody
      • OSCP antibody
      see all

    图片

    • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/ml

      Lane 1 : Recombinant Human ATP5O protein (ab104549) cell lysate at 0.1 µg
      Lane 2 : Recombinant Human ATP5O protein (ab104549) cell lysate at 0.5 µg

      Performed under reducing conditions.

      Predicted band size: 23 kDa
      Observed band size: 24 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-ATP5O antibody [4C11C10D12] staining at 1 ug/ml, shown in green. In Western blot, ab110276 was shown to bind specifically to ATP5O. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

    • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/ml

      Lane 1 : Human heart mitochondria at 5 µg
      Lane 2 : Bovine heart mitochondria at 1 µg

      Predicted band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
    • Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
    • Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
    • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
      All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/ml

      Lane 1 : HDFn (human) cell lysates at 20 µg
      Lane 2 : COS7 (monkey) cell lysates at 20 µg
      Lane 3 : H4IIE (rat) cell lysates at 20 µg
      Lane 4 : MEF (mouse) cell lysates at 20 µg
      Lane 5 : bovine heart mitochondria lysates at 5 µg

      Predicted band size: 23 kDa



      Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

    文献 (25)

    发表研究结果有使用 ab110276？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab110276 被引用在 25 文献中.

    • Cannino G  et al. The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation. Cell Death Differ 29:2335-2346 (2022). PubMed: 35614131
    • Yang W  et al. Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction. Int J Biol Sci 18:2329-2344 (2022). PubMed: 35414767
    • Zhang Y  et al. Sirtuin 3 regulates mitochondrial protein acetylation and metabolism in tubular epithelial cells during renal fibrosis. Cell Death Dis 12:847 (2021). PubMed: 34518519
    • Chen C  et al. Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease. NPJ Parkinsons Dis 7:39 (2021). PubMed: 33980828
    • Liang JR  et al. A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Cell 180:1160-1177.e20 (2020). PubMed: 32160526
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    Immunoprecipitation abreview for Anti-ATP5O antibody [4C11C10D12]

     Excellent
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Bovine papillomavirus Tissue lysate - whole (heart)
    Total protein in input
    5 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    heart
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Xiang Yin

    Verified customer

    提交于 Jun 19 2017

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