AB110276

Anti-ATP5O抗体[4C11C10D12]

Name: ATP5O抗体[4C11C10D12] (ab110276)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110276
Price: 4639 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-ATP5O antibody [4C11C10D12]

(1 Review)

(31 Publications)

Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 31 publications.

查看别名

ATP5O, ATPO, ATP5PO, ATP synthase subunit O, Oligomycin sensitivity conferral protein, OSCP

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Flow Cyt

Unknown

Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276)

HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Unknown

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mL

Lane 1:

Human heart mitochondria at 5 µg

Lane 2:

Bovine heart mitochondria at 1 µg

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Secondary antibody : Goat-Anti-Mouse-IR800 1 : 4000

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

Lane 1:

HDFn (human) cell lysates at 20 µg

Lane 2:

COS7 (monkey) cell lysates at 20 µg

Lane 3:

H4IIE (rat) cell lysates at 20 µg

Lane 4:

MEF (mouse) cell lysates at 20 µg

Lane 5:

bovine heart mitochondria lysates at 5 µg

Predicted band size: 23 kDa

false

Lab

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

False colour image of Western blot : Anti-ATP5O antibody [4C11C10D12] staining at 1 ug/ml, shown in green. In Western blot, ab110276 was shown to bind specifically to ATP5O. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

Lane 1:

Recombinant Human ATP5O protein (<a href='/products/proteins-peptides/recombinant-human-atp5o-protein-ab104549'>ab104549</a>) cell lysate at 0.1 µg

Lane 2:

Recombinant Human ATP5O protein (<a href='/products/proteins-peptides/recombinant-human-atp5o-protein-ab104549'>ab104549</a>) cell lysate at 0.5 µg

Predicted band size: 23 kDa

Observed band size: 24 kDa

false

不同偶联物与剂型 (1)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATP5O antibody [4C11C10D12]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4C11C10D12

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Cow, Human, African green monkey

应用

Flow Cyt, WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization." }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

性能和储存信息

形式

Liquid

纯度

IgG fraction

纯化说明

Near homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP5O also known as OLIGOMYCIN SENSITIVITY CONFERRING PROTEIN (OSCP) plays an important role mechanically in the function of mitochondria. It is a component of the F1F0 ATP synthase complex responsible for the generation of ATP from ADP using the proton gradient across the mitochondrial membrane. ATP5O assists in the stabilization of the enzyme structure. The target protein has a molecular mass of approximately 23 kDa. Expression of ATP5O occurs in various tissues especially in muscles and organs with high energy demands such as the heart and liver.

Biological function summary

ATP5O assists in the energy production process by associating with the mitochondrial ATP synthase complex. It interacts closely with other subunits to form the stator stalk which is vital in the maintenance of the enzyme’s structural integrity during ATP synthesis. The proper function of ATP5O ensures effective conversion of the chemiosmotic energy into chemical energy within the mitochondria making metabolism efficient and supporting cellular energy requirements. This makes its role in energy homeostasis invaluable.

Pathways

ATP5O integrates into oxidative phosphorylation a pathway critical for cellular respiration and energy production. It collaborates with other ATP synthase subunits as well as cytochrome c to complete the electron transport chain culminating the release of ATP for cellular function. ATP5O’s activity impacts cellular energy homeostasis and interacts with proteins like ATP5A and ATP5B which are also part of the ATP synthase complex. Its involvement ensures effective energy transfer and metabolic adaptation to cellular demands.

ATP5O disruptions have links to mitochondrial disorders and neurodegenerative diseases. Mutations affecting ATP5O function can lead to compromised ATP production resulting in conditions like mitochondrial encephalomyopathy. Additionally dysfunctions in ATP5O may contribute to Alzheimer’s disease due to impaired energy metabolism. The link through disease pathways is often closely associated with proteins in the ATP synthase complex highlighting ATP5O's significance in maintaining mitochondrial and neuronal health.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).

See full target information ATP5PO

style

left-column

文献 (31)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 18:2317-2330 PubMed40672059

2025

Metformin Activation of Sirtuin 3 Signaling Regulates Mitochondrial Function Improves Diabetes-Associated Cognitive Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Fei An,Hang Su,Chun-Qiang Zhang,Xue-Ting Wang,Guang-Qiong Zhang,Ling-Yun Fu,Yi-Ni Xu,Ling Tao,Xiang-Chun Shen

Communications biology 7:1486 PubMed39528709

2024

N-terminal cleavage of cyclophilin D boosts its ability to bind F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Coluccino,Alessandro Negro,Antonio Filippi,Camilla Bean,Valentina Pia Muraca,Clarissa Gissi,Diana Canetti,Maria Chiara Mimmi,Elisa Zamprogno,Francesco Ciscato,Laura Acquasaliente,Vincenzo De Filippis,Marina Comelli,Michela Carraro,Andrea Rasola,Christoph Gerle,Paolo Bernardi,Alessandra Corazza,Giovanna Lippe

Biochimica et biophysica acta. Molecular basis of disease 1870:167131 PubMed38521420

2024

A stagewise response to mitochondrial dysfunction in mitochondrial DNA maintenance disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Vincent,Chun Chen,Tiago Bernardino Gomes,Valeria Di Leo,Tuomas Laalo,Kamil Pabis,Rodrick Capaldi,Michael F Marusich,David McDonald,Andrew Filby,Andrew Fuller,Diana Lehmann Urban,Stephan Zierz,Marcus Deschauer,Doug Turnbull,Amy K Reeve,Conor Lawless

Cell death and differentiation 29:2335-2346 PubMed35614131

2022

The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Cannino,Andrea Urbani,Marco Gaspari,Mariaconcetta Varano,Alessandro Negro,Antonio Filippi,Francesco Ciscato,Ionica Masgras,Christoph Gerle,Elena Tibaldi,Anna Maria Brunati,Giorgio Colombo,Giovanna Lippe,Paolo Bernardi,Andrea Rasola

International journal of biological sciences 18:2329-2344 PubMed35414767

2022

Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Kenan Zhang,Zedan Zhang,Jingcheng Zhou,Lei Li,Yawei Xu,Jianhui Qiu,Lin Cai,Yanqing Gong,Kan Gong

Cell death & disease 12:847 PubMed34518519

2021

Sirtuin 3 regulates mitochondrial protein acetylation and metabolism in tubular epithelial cells during renal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Ping Wen,Jing Luo,Hao Ding,Hongdi Cao,Weichun He,Ke Zen,Yang Zhou,Junwei Yang,Lei Jiang

Nature communications 12:4835 PubMed34376679

2021

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Carrer,Ludovica Tommasin,Justina Šileikytė,Francesco Ciscato,Riccardo Filadi,Andrea Urbani,Michael Forte,Andrea Rasola,Ildikò Szabò,Michela Carraro,Paolo Bernardi

NPJ Parkinson's disease 7:39 PubMed33980828

2021

Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chen,David McDonald,Alasdair Blain,Ashwin Sachdeva,Laura Bone,Anna L M Smith,Charlotte Warren,Sarah J Pickett,Gavin Hudson,Andrew Filby,Amy E Vincent,Doug M Turnbull,Amy K Reeve

Genomics, proteomics & bioinformatics 18:679-695 PubMed33607292

2021

Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxi Song,Jun Wang,Jingxu Sun,Xiaowan Chen,Jinxin Shi,Zhonghua Wu,Dehao Yu,Fei Zhang,Zhenning Wang

Biochimica et biophysica acta. Molecular cell research 1868:118942 PubMed33359711

2020

TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel.

Applications

Unspecified application

Species

Unspecified reactive species

Hela Bahri,Jeremie Buratto,Manuel Rojo,Jim Paul Dompierre,Bénédicte Salin,Corinne Blancard,Sylvain Cuvellier,Marie Rose,Amel Ben Ammar Elgaaied,Emmanuel Tetaud,Jean-Paul di Rago,Anne Devin,Stéphane Duvezin-Caubet

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP5O抗体[4C11C10D12]