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AB224139

Anti-ATP5J抗体

Anti-ATP5J antibody

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(3 Publications)

Rabbit Polyclonal ATP5J antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5PF aa 1-150.

查看别名

ATP5A, ATP5J, ATPM, ATP5PF, ATPase subunit F6, ATP synthase peripheral stalk subunit F6

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue sections labeling ATP5J with ab224139. Immunohistochemical staining of human lymph node shows strong granular cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling ATP5J with ab224139. Immunohistochemical staining of human skin shows strong granular cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue sections labeling ATP5J with ab224139. Immunohistochemical staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5J antibody (AB224139)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP5J antibody (AB224139)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATP5J (green) using ab224139 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (AB224139)

Paraffin-embedded human liver tissue stained for ATP5J using ab224139 at 1/500 dilution in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ATP5PF aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P18859

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搭配使用产品

Primary Antibodies

AB169540

Anti-Ndufs1 antibody [EPR11521(B)]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (AB169540)

  • 5
  • 2
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB14715

Anti-SDHA antibody [2E3GC12FB2AE2]

KO Validated

Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (AB14715)

  • 5
  • 25
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP5J also known as ATP synthase subunit F6 is a part of the mitochondrial ATP synthase complex. The ATP5J protein has a mass of about 11 kDa. It is expressed mainly in the inner mitochondrial membrane where it plays an integral role in the process of synthesizing ATP from ADP and inorganic phosphate. ATP5J interacts directly with other subunits of the ATP synthase complex contributing to the functional structure of this vital enzyme complex.
Biological function summary

ATP5J functions in tandem with other components of the ATP synthase complex to facilitate energy production in cells. This complex also known as Complex V catalyzes the final step in oxidative phosphorylation a critical process for cellular energy generation. ATP5J ensures the proper assembly and structural integrity of the ATP synthase complex playing a role in the efficient conversion of the proton motive force into mechanical energy which drives the production of ATP.

Pathways

ATP5J is central to oxidative phosphorylation and ATP synthesis pathways. It interacts closely with key proteins like ATP5A and ATP5B within this pathway. These interactions enable the transduction of electrochemical gradients into usable cellular energy. Furthermore ATP5J's role ensures that ATP synthase maintains its efficiency in energy conversion which is important for meeting cellular metabolic demands.

Mutations or malfunctions of ATP5J can contribute to mitochondrial diseases such as mitochondrial encephalomyopathy. Dysregulation of ATP5J might also relate to disorders like Leigh syndrome where defects in oxidative phosphorylation are common. Other proteins such as COX5A and NDUFS6 also play roles in these conditions highlighting the interconnected nature of mitochondrial function and its impact on human health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Subunit F6, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).
See full target information ATP5PF
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1185 PubMed34475975

2021

Detection of mitochondrial coupling factor 6 in placental tissues from preeclamptic pregnancies and its influence on biological behavior of trophoblast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyu Song,Xufeng Shi,Kan Liu,Ranhong Li,Leilei Niu,Lijun Su,Haiying Wu

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33431668

2021

ATRX and RECQ5 define distinct homologous recombination subpathways.

Applications

Unspecified application

Species

Unspecified reactive species

Amira Elbakry,Szilvia Juhász,Ki Choi Chan,Markus Löbrich

Cancer research 80:524-535 PubMed31690671

2019

Acquired Resistance to HER2-Targeted Therapies Creates Vulnerability to ATP Synthase Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Gale,Yao Li,Jian Cao,Zongzhi Z Liu,Marissa A Holmbeck,Meiling Zhang,Sabine M Lang,Lizhen Wu,Mariana Do Carmo,Swati Gupta,Keisuke Aoshima,Michael P DiGiovanna,David F Stern,David L Rimm,Gerald S Shadel,Xiang Chen,Qin Yan
View all publications
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