Anti-ATP5I抗体(ab122241)
Key features and details
- Rabbit polyclonal to ATP5I
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ATP5I抗体 -
描述
兔多克隆抗体to ATP5I -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence:
RRIAAEEKKKQDELKRIARELAEDDSILK
, corresponding to amino acids 41-69 of Human ATP5I. -
阳性对照
- Human stomach tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates; Human cell line A-431
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常规说明
ab122241 is mono-specific.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
ab122241 is mono-specific. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122241于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. -
序列相似性
Belongs to the ATPase e subunit family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 521 Human
- Omim: 601519 Human
- SwissProt: P56385 Human
- Unigene: 85539 Human
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别名
- ATP 5I antibody
- ATP 5K antibody
- ATP synthase e chain mitochondrial antibody
see all
图片
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Western blot - Anti-ATP5I antibody (ab122241)All lanes : Anti-ATP5I antibody (ab122241) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (ab122241)ab122241, at 1/200 dilution, staining ATP5I in paraffin-embedded Human stomach tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-ATP5I antibody (ab122241)ab122241, at 4µg/ml, staining ATP5I in Human cell line A-431 by Immunofluorescence. Antibody staining is shown in green.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab122241 被引用在 4 文献中.
- Morciano G et al. A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients. Cell Rep 35:108983 (2021). PubMed: 33852870
- Imasawa T et al. Proteomic Study of Low-Birth-Weight Nephropathy in Rats. Int J Mol Sci 22:N/A (2021). PubMed: 34638634
- Carraro M et al. The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore. Cell Rep 32:108095 (2020). PubMed: 32877677
- Mnatsakanyan N et al. A mitochondrial megachannel resides in monomeric F1FO ATP synthase. Nat Commun 10:5823 (2019). PubMed: 31862883