AB97491

Anti-ATP5D 抗体

Name: ATP5D抗体 (ab97491)| Abcam中文官网
Brand: Abcam Limited
SKU: AB97491
Price: 4514 CNY
Availability: InStock

Anti-ATP5D antibody

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(8 Publications)

Rabbit Polyclonal ATP5D antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5F1D aa 1 to C-terminus.

查看别名

ATP5D, ATP5F1D, ATP synthase F1 subunit delta, F-ATPase delta subunit

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (AB97491)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (AB97491)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human breast cancer tissue, stained with ab97491 at 1/250.

Unknown

Western blot - Anti-ATP5D antibody (AB97491)

12% SDS PAGE

All lanes:

Western blot - Anti-ATP5D antibody (ab97491) at 1/500 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Lane 3:

Raji whole cell lysate at 30 µg

Predicted band size: 17 kDa

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Supplier Data

Western blot - Anti-ATP5D antibody (AB97491)

All lanes:

Western blot - Anti-ATP5D antibody (ab97491) at 1/1000 dilution

All lanes:

Mouse brain at 50 µg

Predicted band size: 17 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human ATP5F1D aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30049

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP5D also known as ATP synthase subunit delta is a part of the mitochondrial ATP synthase complex. This protein plays a role in the conversion of ADP to ATP by facilitating proton flow across mitochondrial membranes. ATP5D has a molecular mass of approximately 18-20 kDa. Expression of ATP5D occurs in tissues with high energy demands such as the heart skeletal muscle and liver reflecting its role in energy metabolism.

Biological function summary

ATP5D contributes to the production of cellular energy by acting as an essential subunit in the F1 region of the F1Fo ATP synthase complex. This complex is responsible for ATP synthesis during oxidative phosphorylation. ATP5D's role is critical for maintaining the structural integrity and function of the ATP synthase complex. Its presence ensures efficient ATP production essential for various cellular processes.

Pathways

ATP5D functions within oxidative phosphorylation and energy metabolism pathways. It is closely associated with other ATP synthase subunits required for the ATP production process. Complex interactions include interactions with proteins such as ATP5A and ATP5B within the ATP synthase complex which work together in the energy production pathway essential for cell survival.

Defects or mutations in ATP5D can be related to mitochondrial diseases like mitochondrial encephalomyopathy. Alterations in its normal function can lead to improper cellular energy production. Additionally ATP5D mutations may impact disorders like Leigh syndrome where mitochondrial dysfunction plays a critical role. Understanding ATP5D's connections including those with proteins involved in these disorders helps unravel its impact on these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Subunit delta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable) (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the central stalk subunit gamma, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate (PubMed : 29499186).

See full target information ATP5F1D

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2022:8275574 PubMed36340269

2022

Transcription Factor FXR Activates DHRS9 to Inhibit the Cell Oxidative Phosphorylation and Suppress Colon Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlai Zhao,Yigang Wang,Yang Wang,Jianchao Gao,Haichao Yang,Xiaotang Wu,Hua Li

Frontiers in pharmacology 12:759027 PubMed35095486

2022

QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Ye,Quan Li,Chun-Shui Pan,Li Yan,Kai Sun,Xiao-Yi Wang,Shu-Qi Yao,Jing-Yu Fan,Jing-Yan Han

iScience 25:103605 PubMed35005550

2022

Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Roseth Aass,Robin Mjelle,Martin H Kastnes,Synne S Tryggestad,Luca M van den Brink,Ingrid Aass Roseth,Marita Westhrin,Muhammad Zahoor,Siv H Moen,Tonje M Vikene Nedal,Glenn Buene,Kristine Misund,Anne-Marit Sponaas,Qianli Ma,Anders Sundan,Richard Wj Groen,Tobias S Slørdahl,Anders Waage,Therese Standal

Life science alliance 3: PubMed32788226

2020

MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Ruchika Anand,Arun Kumar Kondadi,Jana Meisterknecht,Mathias Golombek,Oliver Nortmann,Julia Riedel,Leon Peifer-Weiß,Nahal Brocke-Ahmadinejad,David Schlütermann,Björn Stork,Thomas O Eichmann,Ilka Wittig,Andreas S Reichert

Gastroenterology 157:1615-1629.e17 PubMed31446059

2019

New High-Throughput Screening Identifies Compounds That Reduce Viability Specifically in Liver Cancer Cells That Express High Levels of SALL4 by Inhibiting Oxidative Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Justin L Tan,Feng Li,Joanna Z Yeo,Kol Jia Yong,Mahmoud A Bassal,Guo Hao Ng,May Yin Lee,Chung Yan Leong,Hong Kee Tan,Chan-Shuo Wu,Bee Hui Liu,Tim H Chan,Zi Hui Tan,Yun Shen Chan,Siyu Wang,Zhi Han Lim,Tan Boon Toh,Lissa Hooi,Kia Ngee Low,Siming Ma,Nikki R Kong,Alicia J Stein,Yue Wu,Matan T Thangavelu,Atsushi Suzuki,Giridharan Periyasamy,John M Asara,Yock Young Dan,Glenn K Bonney,Edward K Chow,Guo-Dong Lu,Huck Hui Ng,Yoganathan Kanagasundaram,Siew Bee Ng,Wai Leong Tam,Daniel G Tenen,Li Chai

Epigenetics & chromatin 12:44 PubMed31315653

2019

Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Parijat Senapati,Hiroyuki Kato,Michael Lee,Amy Leung,Christine Thai,Angelica Sanchez,Emily J Gallagher,Derek LeRoith,Victoria L Seewaldt,David K Ann,Dustin E Schones

Nature cell biology 19:626-638 PubMed28504707

2017

Regulation of mitochondrial biogenesis in erythropoiesis by mTORC1-mediated protein translation.

Applications

Species

Unspecified reactive species

Xin Liu,Yuannyu Zhang,Min Ni,Hui Cao,Robert A J Signer,Dan Li,Mushan Li,Zhimin Gu,Zeping Hu,Kathryn E Dickerson,Samuel E Weinberg,Navdeep S Chandel,Ralph J DeBerardinis,Feng Zhou,Zhen Shao,Jian Xu

Amyotrophic lateral sclerosis & frontotemporal degeneration 18:210-220 PubMed27899032

2016

Proteomic profiling of the spinal cord in ALS: decreased ATP5D levels suggest synaptic dysfunction in ALS pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jooyeon Engelen-Lee,Anna M Blokhuis,Wim G M Spliet,R Jeroen Pasterkamp,Eleonora Aronica,Jeroen A A Demmers,Roel Broekhuizen,Giovanni Nardo,Niels Bovenschen,Leonard H Van Den Berg

View all publications

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Anti-ATP5D 抗体

Anti-ATP5D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (AB97491)

Western blot - Anti-ATP5D antibody (AB97491)

Western blot - Anti-ATP5D antibody (AB97491)