Anti-ATP5C1抗体[2A1AA11] (ab119686)
![Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.cn/ps/products/119/ab119686/Images/ab119686-132838-anti-atpg-antibody-2a1aa11-western-blot.jpg "Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)")
Key features and details
- Mouse monoclonal [2A1AA11] to ATP5C1
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
-
产品名称
Anti-ATP5C1抗体[2A1AA11]
参阅全部 ATP5C1 一抗 -
描述
小鼠单克隆抗体[2A1AA11] to ATP5C1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
2A1AA11 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
靶标
-
功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
组织特异性
Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta. -
序列相似性
Belongs to the ATPase gamma chain family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 327668 Cow
- Entrez Gene: 509 Human
- Entrez Gene: 11949 Mouse
- Entrez Gene: 116550 Rat
- Omim: 108729 Human
- SwissProt: P05631 Cow
- SwissProt: P36542 Human
- SwissProt: Q91VR2 Mouse
see all -
别名
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
see all
图片
-
Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686)All lanes : Anti-ATP5C1 antibody [2A1AA11] (ab119686) at 1 µg/ml
Lane 1 : human heart homogenate lysate at 15 µg
Lane 2 : human HepG2 cell lysate at 15 µg
Lane 3 : human liver mitochondria lysate at 7.5 µg
Lane 4 : rat liver mitochondria lysate at 7.5 µg
Lane 5 : mouse liver mitochondria lysate at 7.5 µg
Lane 6 : bovine heart mitochondria lysate at 7.5 µg
Secondary
All lanes : Goat anti-mouse HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.cn/ps/products/119/ab119686/Images/ab119686-132837-anti-atpg-antibody-2a1aa11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (ab119686)Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria. -
![Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.cn/ps/products/119/ab119686/Images/ab119686-132836-anti-atpg-antibody-2a1aa11-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (ab119686)Flow cytometry. Hela cells were stained with 1µg/mL anti-ATP5C1 antibody (ab119686) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (ab119686)](https://www.abcam.cn/ps/products/119/ab119686/Images/ab119686-132835-anti-atpg-antibody-2a1aa11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (ab119686)IHC image of ATP5C1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119686, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab119686 被引用在 7 文献中.
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
- Wüst S et al. Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by miR-1/133a-Mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster. Cell Metab 27:1026-1039.e6 (2018). PubMed: 29606596
- Krus U et al. Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells. Biochem J 429:205-13 (2010). PubMed: 20415663
- Fogal V et al. Mitochondrial p32 protein is a critical regulator of tumor metabolism via maintenance of oxidative phosphorylation. Mol Cell Biol 30:1303-18 (2010). PubMed: 20100866
- Stenson BM et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). PubMed: 19556420
- Sharma P et al. Role of pyruvate dehydrogenase complex in traumatic brain injury and Measurement of pyruvate dehydrogenase enzyme by dipstick test. J Emerg Trauma Shock 2:67-72 (2009). PubMed: 19561963
- Ristorcelli E et al. Human tumor nanoparticles induce apoptosis of pancreatic cancer cells. FASEB J 22:3358-69 (2008). PubMed: 18511551