重组Anti-ATP5A抗体[EPR13030(B)] (ab176569)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13030(B)] to ATP5A
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ATP5A抗体[EPR13030(B)]
参阅全部 ATP5A 一抗 -
描述
兔单克隆抗体[EPR13030(B)] to ATP5A -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human ATP5A aa 200-300 (Cysteine residue). The exact sequence is proprietary. Isoform 1
Database link: P25705 -
阳性对照
- HepG2, HeLa, fetal liver and fetal lung lysates; Human liver and fetal heart tissues; HeLa and MCF7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13030(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-ATP5A antibody [EPR13030(B)] (ab195891)
- Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] (ab196198)
- Alexa Fluor® 488 Anti-ATP5A antibody [EPR13030(B)] (ab196467)
- Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] (ab216385)
- Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (ab231692)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176569于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (1) |
1/100 - 1/250.
|
IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended. |
|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended. |
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. -
组织特异性
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
- SwissProt: P15999 Rat
- Unigene: 298280 Human
see all -
别名
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
图片
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All lanes : Anti-ATP5A antibody [EPR13030(B)] (ab176569) at 0.01 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
Ab176569 (purified) staining ATP5A in HeLa (human cervix adenocarcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (4.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HeLa cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP5A with purified ab176569 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-ATP5A antibody [EPR13030(B)] (ab176569) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa -
Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 (unpurified) at a 1/100 dilution (green). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 (unpurified) at a 1/10 dilution (red) or a rabbit IgG negative control (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab176569 被引用在 23 文献中.
- Jang S et al. Beta-Amyloid Instigates Dysfunction of Mitochondria in Cardiac Cells. Cells 11:N/A (2022). PubMed: 35159183
- Zhu Z et al. Photobiomodulation promotes repair following spinal cord injury by restoring neuronal mitochondrial bioenergetics via AMPK/PGC-1α/TFAM pathway. Front Pharmacol 13:991421 (2022). PubMed: 36172183
- Li S et al. Reduced PGC-1β protein expression may underlie corticosterone inhibition of mitochondrial biogenesis and oxidative phosphorylation in chicken muscles. Front Physiol 13:989547 (2022). PubMed: 36311241
- Waldhart AN et al. Excess dietary carbohydrate affects mitochondrial integrity as observed in brown adipose tissue. Cell Rep 36:109488 (2021). PubMed: 34348139
- Li Y et al. CISD3 inhibition drives cystine-deprivation induced ferroptosis. Cell Death Dis 12:839 (2021). PubMed: 34497268