Anti-ATP5A抗体(ab222887)
Key features and details
- Rabbit polyclonal to ATP5A
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ATP5A抗体
参阅全部 ATP5A 一抗 -
描述
兔多克隆抗体to ATP5A -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ATP5A aa 44-257.
Database link: P25705 -
阳性对照
- WB: HeLa, K562, MCF7 and PC-3 whole cell lysates; Mouse heart lysate. IHC-P: Human liver and kidney tissues. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| IP |
1/200 - 1/2000.
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| WB |
1/1000 - 1/5000. Detects a band of approximately 60, 58 kDa (predicted molecular weight: 60 kDa).
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| 说明 |
|---|
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IHC-P
1/20 - 1/200. |
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IP
1/200 - 1/2000. |
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WB
1/1000 - 1/5000. Detects a band of approximately 60, 58 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. -
组织特异性
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Omim: 164360 Human
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
- Unigene: 298280 Human
- Unigene: 276137 Mouse
- Unigene: 474505 Mouse
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别名
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
图片
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Western blot - Anti-ATP5A antibody (ab222887)All lanes : Anti-ATP5A antibody (ab222887) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 5 : Mouse heart lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 58,60 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)Paraffin-embedded human liver tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)Paraffin-embedded human kidney tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-ATP5A antibody (ab222887)ATP5A was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222887 at 1/200 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab222887 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab222887 尚未被引用在任何文献中。