Anti-ATP5A抗体[7H10BD4F9] (ab110273)
![Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.cn/ps/products/110/ab110273/Images/ab110273-123468-anti-atp5a-antibody-7h10bd4f9-western-blot.gif "Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)")
Key features and details
- Mouse monoclonal [7H10BD4F9] to ATP5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
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产品名称
Anti-ATP5A抗体[7H10BD4F9]
参阅全部 ATP5A 一抗 -
描述
小鼠单克隆抗体[7H10BD4F9] to ATP5A -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Full length protein corresponding to Cow ATP5A.
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阳性对照
- Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Near homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
7H10BD4F9 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110273于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. -
组织特异性
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 282578 Cow
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P19483 Cow
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
see all -
别名
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
see all
图片
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Western blot - Anti-ATP5A antibody [7H10BD4F9] (ab110273)All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 60 kDa -
![Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.cn/ps/products/110/ab110273/Images/ab110273-123467-anti-atp5a-antibody-7h10bd4f9-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] (ab110273)HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)](https://www.abcam.cn/ps/products/110/ab110273/Images/ab110273-123466-anti-atp5a-antibody-7h10bd4f9-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] (ab110273)Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
数据表及文件
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SDS download
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Datasheet download
文献 (80)
ab110273 被引用在 80 文献中.
- Skopkova M et al. ATAD3A-related pontocerebellar hypoplasia: new patients and insights into phenotypic variability. Orphanet J Rare Dis 18:92 (2023). PubMed: 37095554
- Scoma ER et al. Human Prune Regulates the Metabolism of Mammalian Inorganic Polyphosphate and Bioenergetics. Int J Mol Sci 24:N/A (2023). PubMed: 37762163
- Pereira SP et al. Characterizing Early Cardiac Metabolic Programming via 30% Maternal Nutrient Reduction during Fetal Development in a Non-Human Primate Model. Int J Mol Sci 24:N/A (2023). PubMed: 37894873
- Zhang Z et al. Ruthenium 360 and mitoxantrone inhibit mitochondrial calcium uniporter channel to prevent liver steatosis induced by high-fat diet. Br J Pharmacol 179:2678-2696 (2022). PubMed: 34862596
- Nguyen VVT et al. A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles. J Extracell Vesicles 11:e12280 (2022). PubMed: 36382606