重组Anti-ATP4A抗体[EPR12251] (ab174293)
![Western blot - Anti-ATP4A antibody [EPR12251] (ab174293)](https://www.abcam.cn/ps/products/174/ab174293/Images/ab174293-199380-anti-atp4a-antibody-epr12251-western-blot.jpg "Western blot - Anti-ATP4A antibody [EPR12251] (ab174293)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12251] to ATP4A
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-ATP4A抗体[EPR12251]
参阅全部 ATP4A 一抗 -
描述
兔单克隆抗体[EPR12251] to ATP4A -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment within Human ATP4A. The exact sequence is proprietary.
Database link: P20648 -
阳性对照
- Human stomach and rat stomach lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12251 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174293于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 114 kDa. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
-
功能
Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. -
组织特异性
Found in gastric mucosa. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 495 Human
- Entrez Gene: 24216 Rat
- Omim: 137216 Human
- SwissProt: P20648 Human
- SwissProt: P09626 Rat
- Unigene: 36992 Human
-
别名
- Atp4a antibody
- ATP4A_HUMAN antibody
- ATP6A antibody
see all
图片
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Western blot - Anti-ATP4A antibody [EPR12251] (ab174293)All lanes : Anti-ATP4A antibody [EPR12251] (ab174293) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Rat stomach lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 114 kDa -
![Anti-ATP4A antibody [EPR12251] (ab174293)](https://www.abcam.cn/ps/products/174/ab174293/Images/ab174293-2-benefits-of-recombinant-antibodies.png)
Anti-ATP4A antibody [EPR12251] (ab174293)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab174293 被引用在 1 文献中.
- Paresi CJ et al. Benzimidazole covalent probes and the gastric H(+)/K(+)-ATPase as a model system for protein labeling in a copper-free setting. Mol Biosyst 12:1772-80 (2016). PubMed: 26952080