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AB8344

Anti-ATP1B1抗体[464.8]

Anti-ATP1B1 antibody [464.8]

4

(1 Review)

|

(12 Publications)

Mouse Monoclonal ATP1B1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 12 publications.

查看别名

ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1

2 Images
Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)

Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-ATP1B1 antibody [464.8] (AB8344)
  • WB

Ap1152848****

Western blot - Anti-ATP1B1 antibody [464.8] (AB8344)

All lanes:

Western blot - Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution

All lanes:

Human kidney tissue lysate - total protein (<a href='/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Predicted band size: 35 kDa

Observed band size: 45 kDa

true

Exposure time: 4min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

464.8

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.
Biological function summary

ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.

Pathways

ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.

ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed : 19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed : 34011520).. Involved in cell adhesion and establishing epithelial cell polarity.
See full target information ATP1B1
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

European journal of oral sciences 122:21-6 PubMed24313748

2013

Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wen,Rodrigo S Lacruz,Charles E Smith,Michael L Paine

PloS one 8:e70750 PubMed23976956

2013

Ca²⁺/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures.

Applications

WB

Species

Rat

Qing Lu,Valerie A Harris,Xutong Sun,Yali Hou,Stephen M Black

Gut 63:808-17 PubMed23916961

2013

Farnesoid X receptor agonists attenuate colonic epithelial secretory function and prevent experimental diarrhoea in vivo.

Applications

WB

Species

Mouse

Magdalena S Mroz,Niamh Keating,Joseph B Ward,Rafiquel Sarker,Silvie Amu,Gabriella Aviello,Mark Donowitz,Padraic G Fallon,Stephen J Keely

Free radical biology & medicine 53:1139-51 PubMed22728269

2012

Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.

Applications

WB

Species

Unspecified reactive species

Qing Lu,Mark S Wainwright,Valerie A Harris,Saurabh Aggarwal,Yali Hou,Thomas Rau,David J Poulsen,Stephen M Black

EMBO molecular medicine 2:63-75 PubMed20091762

2010

Role of the WNK-activated SPAK kinase in regulating blood pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Fatema H Rafiqi,Annie Mercier Zuber,Mark Glover,Ciaran Richardson,Stewart Fleming,Sofija Jovanović,Aleksandar Jovanović,Kevin M O'Shaughnessy,Dario R Alessi

Reproductive sciences (Thousand Oaks, Calif.) 15:366-73 PubMed18325929

2008

Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat.

Applications

Unspecified application

Species

Unspecified reactive species

Mark G Neerhof,Tamas Jilling,Sylvia Synowiec,Saira Khan,Larry G Thaete

European journal of immunology 37:1194-203 PubMed17407098

2007

NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Donini,Elena Zenaro,Nicola Tamassia,Stefano Dusi

The American journal of physiology 267:F75-85 PubMed8048568

1994

Expression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

S K Van Why,A S Mann,T Ardito,N J Siegel,M Kashgarian

The American journal of physiology 266:C579-89 PubMed8166221

1994

Analysis of subunit assembly of the Na-K-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

D M Fambrough,M V Lemas,M Hamrick,M Emerick,K J Renaud,E M Inman,B Hwang,K Takeyasu

The Journal of biological chemistry 268:14342-7 PubMed8390991

1993

Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly.

Applications

Unspecified application

Species

Unspecified reactive species

C J Gottardi,M J Caplan
View all publications
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