重组Anti-ATP citrate lyase (phospho T447 + S451)抗体[EP737Y] (ab53007)
![Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)](https://www.abcam.cn/ps/products/53/ab53007/Images/ab53007-383298-anti-atp-citrate-lyase-phospho-t447-s451-antibody-ep737y-westernblot-human-mouse.jpg "Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ATP citrate lyase (phospho T447 + S451)抗体[EP737Y]
参阅全部 ATP citrate lyase 一抗 -
描述
兔单克隆抗体[EP737Y] to ATP citrate lyase (phospho T447 + S451) -
宿主
Rabbit -
特异性
ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Untreated and insulin treated NIH/3T3, NIH/3T3, HeLa and C6 whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP737Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53007于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 122 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 122 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. -
序列相似性
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain. -
翻译后修饰
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 24159 Rat
- Omim: 108728 Human
- SwissProt: P53396 Human
- SwissProt: Q91V92 Mouse
- SwissProt: P16638 Rat
- Unigene: 387567 Human
see all -
别名
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
图片
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Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 15 minutes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)](https://www.abcam.cn/ps/products/53/ab53007/Images/ab53007-383299-anti-atp-citrate-lyase-phospho-t447-s451-antibody-ep737y-westernblot-rat.jpg)
Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng Calyculin A for 60 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)](https://www.abcam.cn/ps/products/53/ab53007/Images/ab53007-272492-anti-atp-citrate-lyase-phospho-t447-s451-antibody-ep737y-western-blot.jpg)
Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (unpurified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysate
Lane 2 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 122 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)](https://www.abcam.cn/ps/products/53/ab53007/Images/ab53007-6-benefits-of-recombinant-antibodies.png)
Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab53007 被引用在 4 文献中.
- García-Arroyo FE et al. Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats. Biomolecules 9:N/A (2019). PubMed: 31614639
- Zhou Y et al. Hepatocellular carcinoma-derived exosomal miRNA-21 contributes to tumor progression by converting hepatocyte stellate cells to cancer-associated fibroblasts. J Exp Clin Cancer Res 37:324 (2018). PubMed: 30591064
- Grunert T et al. A comparative proteome analysis links tyrosine kinase 2 (Tyk2) to the regulation of cellular glucose and lipid metabolism in response to poly(I:C). J Proteomics 74:2866-80 (2011). WB . PubMed: 21787891
- Chu KY et al. ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells. J Biol Chem 285:32606-15 (2010). PubMed: 20693577