Anti-ATP-binding cassette sub-family A member 3抗体[3C9] (ab24751)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)](https://www.abcam.cn/ps/products/24/ab24751/Images/ab24751-5-antiatpbindingcassettesubfamilyamember3antibody3c9immunohistochemistrylungmouse.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)")
Key features and details
- Mouse monoclonal [3C9] to ATP-binding cassette sub-family A member 3
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2a
概述
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产品名称
Anti-ATP-binding cassette sub-family A member 3抗体[3C9]
参阅全部 ATP-binding cassette sub-family A member 3 一抗 -
描述
小鼠单克隆抗体[3C9] to ATP-binding cassette sub-family A member 3 -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: FFPE rat lung and mouse lung tissue sections.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
3C9 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24751于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol. -
组织特异性
Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma. -
疾病相关
Defects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. -
序列相似性
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
结构域
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 27410 Mouse
- Entrez Gene: 302973 Rat
- SwissProt: Q8R420 Mouse
- SwissProt: P16970 Rat
- Unigene: 239470 Mouse
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别名
- ABC 3 antibody
- ABC C antibody
- ABC C transporter antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)Lab
IHC image of ATP-binding cassette sub-family A member 3 staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24751, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)](https://www.abcam.cn/ps/products/24/ab24751/Images/ab24751-35602-anti-atp-binding-cassette-sub-family-a-member-3-antibody-3c9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)IHC image of P180 Lamellar Body Protein staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24751, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab24751 被引用在 23 文献中.
- Sucre JM et al. Alveolar repair following LPS-induced injury requires cell-ECM interactions. JCI Insight 8:N/A (2023). PubMed: 37279065
- Luan Y et al. Pazopanib Is a Potential Treatment for Coronavirus-Induced Lung Injuries. J Immunol 209:723-730 (2022). PubMed: 35914834
- Li X et al. The Role of Autophagy in Lamellar Body Formation and Surfactant Production in Type 2 Alveolar Epithelial Cells. Int J Biol Sci 18:1107-1119 (2022). PubMed: 35173542
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Li T et al. OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis. Oxid Med Cell Longev 2021:4158495 (2021). PubMed: 34426759