AB24751

Anti-ATP-binding cassette sub-family A member 3抗体[3C9]

Name: ATP-binding cassette sub-family A member 3抗体[3C9] (ab24751)| Abcam中文官网
Brand: Abcam Limited
SKU: AB24751
Price: 1493 CNY
Availability: InStock
Rating: 4 (9 reviews)

Anti-ATP-binding cassette sub-family A member 3 antibody [3C9]

BOND RX™ Validated
了解详情

(9 Reviews)

(26 Publications)

Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751) is a mouse monoclonal antibody detecting ATP-binding cassette sub-family A member 3 in IHC-P. Suitable for Mouse, Rat.

- Over 20 publications
- Trusted since 2005

查看别名

Phospholipid-transporting ATPase ABCA3, ATP-binding cassette sub-family A member 3, Xenobiotic-transporting ATPase ABCA3, Abca3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (AB24751)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (AB24751)

IHC image of P180 Lamellar Body Protein staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24751, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (AB24751)

IHC image of ATP-binding cassette sub-family A member 3 staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24751, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

3C9

亚型

IgG2a

不含载体蛋白

反应种属

Rat, Mouse

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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产品详情

What is this antibody validated in?
Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Mouse, Rat samples.

Trusted by the scientific community
Anti-ATP-binding cassette sub-family A member 3 [3C9] (ab24751) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists
Anti-ATP-binding cassette sub-family A member 3 [3C9] (ab24751) has over 5 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP-binding cassette sub-family A member 3 (ABCA3) also known as Surfactant Metabolism Dysfunction Pulmonary 3 is a vital membrane protein weighing approximately 170 kDa. It belongs to the ATP-binding cassette (ABC) protein family which also includes family binding proteins that transport various molecules across cellular membranes. ABCA3 is mainly expressed in alveolar type II cells of the lung where it plays a significant mechanical role in surfactant metabolism. The protein facilitates the transport of lipids into lamellar bodies which are critical for surfactant storage and secretion.

Biological function summary

ABCA3 is essential for normal pulmonary function as it contributes to surfactant phospholipid homeostasis. It operates as a monomer and is part of a larger complex with other lipid-processing proteins. This interaction ensures the correct assembly and secretion of pulmonary surfactant which reduces surface tension in the alveoli and prevents alveolar collapse. The dysfunction or absence of ABCA3 can disrupt these processes compromising lung function.

Pathways

The role of ABCA3 is significant in the surfactant metabolism pathway and lipid transportation processes. The surfactant metabolism pathway ensures the availability of pulmonary surfactant necessary for efficient respiratory function. In these pathways ABCA3 intersects with other proteins such as ABCA1 and proteins involved in the phospholipid synthesis and transport processes ensuring lipid proteins are correctly assembled for surfactant formation.

ABCA3 mutations associate with respiratory diseases such as Neonatal Respiratory Distress Syndrome and interstitial lung disease. These diseases often result from impaired surfactant production and secretion caused by ABCA3 deficiency. Mutations in this protein can also lead to dysfunctional interactions with partner proteins like Surfactant Protein B (SP-B) and Surfactant Protein C (SP-C) further implicating its role in disease pathology. Understanding the connection between ABCA3 and these proteins reveals potential therapeutic targets for treating associated pulmonary conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant (PubMed : 17142808, PubMed : 17267394, PubMed : 17540762, PubMed : 17577581, PubMed : 20190032, PubMed : 28034695). Transports preferentially phosphatidylcholine containing short acyl chains (PubMed : 17142808). In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity (By similarity).

See full target information Abca3

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文献 (26)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37279065

2023

Alveolar repair following LPS-induced injury requires cell-ECM interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Ms Sucre,Fabian Bock,Nicholas M Negretti,John T Benjamin,Peter M Gulleman,Xinyu Dong,Kimberly T Ferguson,Christopher S Jetter,Wei Han,Yang Liu,Seunghyi Kook,Jason J Gokey,Susan H Guttentag,Jonathan A Kropski,Timothy S Blackwell,Roy Zent,Erin J Plosa

Molecular medicine (Cambridge, Mass.) 29:70 PubMed37226090

2023

Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tong,Danni Li,Na Liu,Wanjie Huang,Xinyi Zhao,Dan Zhang,Xindong Xue,Jianhua Fu

Journal of immunology (Baltimore, Md. : 1950) 209:723-730 PubMed35914834

2022

Pazopanib Is a Potential Treatment for Coronavirus-Induced Lung Injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luan,Qianying Yuan,Qijun Wang,Susan Compton,Dianqing Wu,Wenwen Tang

Scientific reports 12:8742 PubMed35610327

2022

The normal and fibrotic mouse lung classified by spatial proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Ciccimarra,Maddalena M Bolognesi,Matteo Zoboli,Giorgio Cattoretti,Franco F Stellari,Francesca Ravanetti

Journal of visualized experiments : JoVE : PubMed35129166

2022

Engineered Lung Tissues Prepared from Decellularized Lung Slices.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Leiby,Ronald Ng,Stuart G Campbell,Laura E Niklason

International journal of biological sciences 18:1107-1119 PubMed35173542

2022

The Role of Autophagy in Lamellar Body Formation and Surfactant Production in Type 2 Alveolar Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Li,Liang Wang,Jialin Hao,Qingfeng Zhu,Min Guo,Changjing Wu,Sihui Li,Qiqiang Guo,Qiuhong Ren,Ning Bai,Fei Yi,Bo Jiang,Wenyu Zhang,Yanling Feng,Hongde Xu,Han Jiang,Xiaoyue Zhai,Guohua Zhang,Hong-Long Ji,Xuesong Yang,Dan Zhang,Jianhua Fu,Jianjun Chang,Xiaoyu Song,Liu Cao

Oxidative medicine and cellular longevity 2021:4158495 PubMed34426759

2021

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Li,Xin-Yu Yang,Ding-Jie Xu,Zi-Yi Gao,Yi-Bing Gao,Fu-Yu Jin,Ya-Qian Li,Shu-Peng Liu,Shi-Feng Li,Xue-Min Gao,Wen-Chen Cai,Na Mao,Zhong-Qiu Wei,He-Liang Liu,Ying Sun,Fang Yang,Hong Xu

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33990468

2021

AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghyi Kook,Ping Wang,Shufang Meng,Christopher S Jetter,Jennifer M S Sucre,John T Benjamin,Jason J Gokey,Hayley A Hanby,Alexa Jaume,Laura Goetzl,Michael S Marks,Susan H Guttentag

Cell stem cell 28:1775-1789.e5 PubMed33974915

2021

Age-dependent alveolar epithelial plasticity orchestrates lung homeostasis and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ian J Penkala,Derek C Liberti,Joshua Pankin,Aravind Sivakumar,Madison M Kremp,Sowmya Jayachandran,Jeremy Katzen,John P Leach,Rebecca Windmueller,Katharine Stolz,Michael P Morley,Apoorva Babu,Su Zhou,David B Frank,Edward E Morrisey

Cell reports 33:108477 PubMed33296658

2020

Autophagy Is Required for Maturation of Surfactant-Containing Lamellar Bodies in the Lung and Swim Bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Morishita,Yuki Kanda,Takeshi Kaizuka,Haruka Chino,Kazuki Nakao,Yoshimi Miki,Yoshitaka Taketomi,Jun-Lin Guan,Makoto Murakami,Atsu Aiba,Noboru Mizushima

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP-binding cassette sub-family A member 3抗体[3C9]

Anti-ATP-binding cassette sub-family A member 3 antibody [3C9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (AB24751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (AB24751)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原