Anti-ATIC抗体[F38 P7 H9] (ab33520)
Key features and details
- Mouse monoclonal [F38 P7 H9] to ATIC
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-ATIC抗体[F38 P7 H9]
参阅全部 ATIC 一抗 -
描述
小鼠单克隆抗体[F38 P7 H9] to ATIC -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, WBmore details -
种属反应性
与反应: Human
预测可用于: Chicken -
免疫原
Synthetic peptide corresponding to Human ATIC aa 583-592 conjugated to Ovalbumin.
Sequence:AHTNLRLFHH
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阳性对照
- Recombinant Human ATIC protein (ab114743) can be used as a positive control in WB. WB: Hep G2, HCT 116, K-562, COLO 205, NTERA-2 and HT -29 cell lysates. IHC-P: Human colon carcinoma tissue .
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
F38 P7 H9 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 65 kDa.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 65 kDa. |
靶标
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功能
Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. -
通路
Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1. -
疾病相关
Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. -
序列相似性
Belongs to the purH family. -
结构域
The IMP cyclohydrolase activity resides in the N-terminal region. - Information by UniProt
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数据库链接
- Entrez Gene: 396091 Chicken
- Entrez Gene: 471 Human
- Omim: 601731 Human
- SwissProt: P31335 Chicken
- SwissProt: P31939 Human
- Unigene: 90280 Human
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别名
- AICAR formyltransferase/IMP cyclohydrolase antibody
- 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
- 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
see all
图片
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All lanes : Anti-ATIC antibody [F38 P7 H9] (ab33520) at 2 µg/ml
Lane 1 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 5 : NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 6 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATIC antibody [F38 P7 H9] (ab33520)
ab33520, at a concentration of 5 µg/ml, staining ATIC in Human colon carcinoma tissue by Immunohistochemistry.
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Overlay histogram showing HeLa cells stained with ab33520 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33520, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab33520 被引用在 6 文献中.
- Niu N et al. ATIC facilitates cell growth and migration by upregulating Myc expression in lung adenocarcinoma. Oncol Lett 23:131 (2022). PubMed: 35251351
- Li R et al. Upregulation of ATIC in multiple myeloma tissues based on tissue microarray and gene microarrays. Int J Lab Hematol 43:409-417 (2021). PubMed: 33226193
- Parviainen VI et al. Label-free mass spectrometry proteome quantification of human embryonic kidney cells following 24 hours of sialic acid overproduction. Proteome Sci 11:38 (2013). WB . PubMed: 23915316
- Baresova V et al. Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency. Hum Mol Genet 21:1534-43 (2012). ICC/IF ; Human . PubMed: 22180458
- Boccalatte FE et al. The enzymatic activity of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase is enhanced by NPM-ALK: new insights in ALK-mediated pathogenesis and the treatment of ALCL. Blood 113:2776-90 (2009). WB ; Human . PubMed: 18845790
- Gunter JH et al. Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms. Int J Biochem Cell Biol 40:1716-28 (2008). PubMed: 18295529