Anti-ATG4B抗体(ab225882)
Key features and details
- Rabbit polyclonal to ATG4B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ATG4B抗体
参阅全部 ATG4B 一抗 -
描述
兔多克隆抗体to ATG4B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Giant Panda
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免疫原
Synthetic peptide within Human ATG4B aa 350-393. The exact sequence is proprietary. (NP_037457.3).
Database link: Q9Y4P1 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225882 was affinity purified using an epitope specific to ATG4B immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225882于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 44 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. -
组织特异性
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. -
序列相似性
Belongs to the peptidase C54 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 748674 Chimpanzee
- Entrez Gene: 609599 Dog
- Entrez Gene: 101132371 Gorilla
- Entrez Gene: 100067797 Horse
- Entrez Gene: 23192 Human
- Entrez Gene: 66615 Mouse
- Entrez Gene: 100462747 Pig
- Entrez Gene: 316640 Rat
see all -
别名
- APG4 autophagy 4 homolog B antibody
- APG4B antibody
- ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
see all
图片
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Western blot - Anti-ATG4B antibody (ab225882)All lanes : Anti-ATG4B antibody (ab225882) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ATG4B antibody (ab225882)ATG4B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225882 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225882 at 1 µg/ml.
Lane 1: ab225882 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225882 尚未被引用在任何文献中。