重组Anti-ATG4A抗体[EPR4122] (ab108322)
![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.cn/ps/products/108/ab108322/Images/ab108322-453261-anti-atg4a-antibody-epr4122-western-blot-hela-wt-atg4a-knockout-hela-hepg2-daudi-human.jpg "Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4122] to ATG4A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATG4A抗体[EPR4122]
参阅全部 ATG4A 一抗 -
描述
兔单克隆抗体[EPR4122] to ATG4A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal liver, HeLa, Daudi, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4122 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval. |
靶标
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功能
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. -
组织特异性
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. -
序列相似性
Belongs to the peptidase C54 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 115201 Human
- Entrez Gene: 666468 Mouse
- Omim: 300663 Human
- SwissProt: Q8WYN0 Human
- SwissProt: Q8C9S8 Mouse
- Unigene: 8763 Human
- Unigene: 102230 Mouse
- Unigene: 393320 Mouse
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别名
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
see all
图片
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Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG4A knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108322 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab108322 was shown to react with ATG4A in wild-type HeLa cells in Western blot with loss of signal observed in ATG4A knockout cell line ab265738 (ATG4A knockout cell lysate ab257846). Wild-type HeLa and ATG4A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108322 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.cn/ps/products/108/ab108322/Images/ab108322-120888-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.cn/ps/products/108/ab108322/Images/ab108322-120887-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.cn/ps/products/108/ab108322/Images/ab108322-120889-anti-atg4a-antibody-epr4122-western-blot.jpg)
Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.cn/ps/products/108/ab108322/Images/ab108322-4-benefits-of-recombinant-antibodies.png)
Anti-ATG4A antibody [EPR4122] (ab108322)
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab108322 被引用在 12 文献中.
- Li P et al. WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 38003226
- Li Q & Zan L Knockdown of ATG4A inhibits breast cancer progression and promotes tamoxifen chemosensitivity by suppressing autophagy. Mol Med Rep 25:N/A (2022). PubMed: 35088889
- Sun L et al. Deacetylation of ATG4B promotes autophagy initiation under starvation. Sci Adv 8:eabo0412 (2022). PubMed: 35921421
- Xie S et al. miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway. iScience 24:103271 (2021). PubMed: 34761190
- Zhu K et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291