重组Anti-ATG3抗体[EPR4802] (ab108282)
![Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)](https://www.abcam.cn/ps/products/108/ab108282/Images/ab108282-393584-anti-atg3-antibody-epr4802-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4802] to ATG3
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATG3抗体[EPR4802]
参阅全部 ATG3 一抗 -
描述
兔单克隆抗体[EPR4802] to ATG3 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293T, K562, HeLa or Jurkat cell lysates. Flow Cyt (intra): Permeabilized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4802 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108282于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). |
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. -
组织特异性
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. -
序列相似性
Belongs to the ATG3 family. -
翻译后修饰
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
- SwissProt: Q6AZ50 Rat
- Unigene: 477126 Human
see all -
别名
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
see all
图片
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Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATG3 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108282 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108282 Anti-ATG3 antibody [EPR4802] was shown to specifically react with ATG3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266707 (knockout cell lysate ab257363) was used. Wild-type and ATG3 knockout samples were subjected to SDS-PAGE. ab108282 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-ATG3 antibody [EPR4802] (ab108282)](https://www.abcam.cn/ps/products/108/ab108282/Images/ab108282-120791-anti-atg3-antibody-epr4802-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ATG3 antibody [EPR4802] (ab108282)Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).
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![Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)](https://www.abcam.cn/ps/products/108/ab108282/Images/ab108282-120790-anti-atg3-antibody-epr4802-western-blot.jpg)
Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Anti-ATG3 antibody [EPR4802] (ab108282)](https://www.abcam.cn/ps/products/108/ab108282/Images/ab108282-4-benefits-of-recombinant-antibodies.png)
Anti-ATG3 antibody [EPR4802] (ab108282)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108282 被引用在 5 文献中.
- Ren H et al. Equine lentivirus counteracts SAMHD1 restriction by Rev-mediated degradation of SAMHD1 via the BECN1-dependent lysosomal pathway. Autophagy 17:2800-2817 (2021). PubMed: 33172327
- He GQ et al. Associations between Huwe1 and autophagy in rat cerebral neuron oxygen-glucose deprivation and reperfusion injury. Mol Med Rep 22:5083-5094 (2020). PubMed: 33173969
- Agrotis A et al. Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. J Biol Chem 294:12610-12621 (2019). PubMed: 31315929
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196
- He G et al. Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury. Apoptosis 21:390-403 (2016). WB ; Rat . PubMed: 26882903