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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-ATG12抗体(ab4752)

  • Datasheet
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Western blot - Anti-ATG12 antibody (ab4752)

    Key features and details

    • Rabbit polyclonal to ATG12
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-ATG12 antibody [EPR4800] (ab109491)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-ATG12抗体
      参阅全部 ATG12 一抗
    • 描述

      兔多克隆抗体to ATG12
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Saccharomyces cerevisiae
    • 免疫原

      Recombinant full length protein corresponding to Saccharomyces cerevisiae ATG12.

    • 阳性对照

      • Most yeast cell lysates can be used as a positive control without induction or stimulation.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • 纯度

      IgG fraction
    • 纯化说明

      Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ub-like Proteins
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other
      • Cardiovascular
      • Heart
      • Autophagy
      • APG gene products
      • Cancer
      • Signal transduction
      • Autophagy
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Autophagy and mitophagy
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Autophagy and mitophagy
      • APG gene products
      • Cancer
      • Cell Death
      • Autophagy
      • Signal Transduction
      • Cancer
      • Cell Death
      • Autophagy
      • APG gene products

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4752于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/2000. Detects a band of approximately 22.1 kDa.
    说明
    WB
    1/2000. Detects a band of approximately 22.1 kDa.

    靶标

    • 功能

      Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
    • 组织特异性

      Ubiquitous.
    • 序列相似性

      Belongs to the ATG12 family.
    • 结构域

      Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
    • 细胞定位

      Cytoplasm.
    • Target information above from: UniProt accession O94817 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 别名

      • Apg12 antibody
      • APG12-like antibody
      • APG12L antibody
      • ATG12 antibody
      • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
      • ATG12 autophagy related 12 homolog antibody
      • ATG12_HUMAN antibody
      • Autophagy 12 antibody
      • Autophagy-related protein 12 antibody
      • FBR93 antibody
      • HAPG12 antibody
      • Ubiquitin-like protein ATG12 antibody
      see all

    图片

    • Western blot - Anti-ATG12 antibody (ab4752)
      Western blot - Anti-ATG12 antibody (ab4752)

      ab4752 generated by immunization with recombinant Saccharomyces cerevisiae ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.   Panel A shows total protein staining using ponceau.  Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125).  Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090).  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab4752?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4752 被引用在 1 文献中.

    • Crews L  et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . PubMed: 20174468

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    I am interested in your rabbit antibody to Apg12 (ab4752). On the data sheet, it says that it has not been tested in other species. Is there an update on this? Do you know if it will react with human Apg12? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    Sorry but there has been no further information on this and we do not know if it will cross react with human.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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