重组Anti-ATG10抗体[EPR4804] (ab124711)
![Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.cn/ps/products/124/ab124711/Images/ab124711-414933-anti-atg10-antibody-epr4804-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4804] to ATG10
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATG10抗体[EPR4804]
参阅全部 ATG10 一抗 -
描述
兔单克隆抗体[EPR4804] to ATG10 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ATG10 aa 200-300 (C terminal).
Database link: Q9H0Y0 -
阳性对照
- WB: HeLa, K562, and 293T cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 9.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4804 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124711于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
靶标
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功能
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. -
序列相似性
Belongs to the ATG10 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 83734 Human
- Omim: 610800 Human
- SwissProt: Q9H0Y0 Human
- Unigene: 713698 Human
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别名
- Apg 10 antibody
- APG 10L antibody
- APG10 antibody
see all
图片
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Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ATG10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab124711 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124711 Anti-ATG10 antibody [EPR4804] was shown to specifically react with ATG10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265682 (knockout cell lysate ab258786) was used. Wild-type and ATG10 knockout samples were subjected to SDS-PAGE. ab124711 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.cn/ps/products/124/ab124711/Images/ab124711-138513-anti-atg10-antibody-epr4804-western-blot.jpg)
Western blot - Anti-ATG10 antibody [EPR4804] (ab124711)All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa -
![OI-RD Scanning - Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.cn/ps/products/124/ab124711/Images/ab124711-138512-anti-atg10-antibody-epr4804.jpg)
OI-RD Scanning - Anti-ATG10 antibody [EPR4804] (ab124711) -
![Anti-ATG10 antibody [EPR4804] (ab124711)](https://www.abcam.cn/ps/products/124/ab124711/Images/ab124711-4-benefits-of-recombinant-antibodies.png)
Anti-ATG10 antibody [EPR4804] (ab124711)
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab124711 被引用在 14 文献中.
- Peng J et al. The m6A methyltransferase METTL3 affects autophagy and progression of nasopharyngeal carcinoma by regulating the stability of lncRNA ZFAS1. Infect Agent Cancer 17:1 (2022). PubMed: 34980191
- Xu X et al. A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer. Aging (Albany NY) 14:4858-4873 (2022). PubMed: 35680565
- Polo P et al. Robustness of the Autophagy Pathway to Somatic Copy Number Losses. Cells 11:N/A (2022). PubMed: 35681458
- Pavel M et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
- Zhu Y et al. SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by ß-catenin and Beclin1/autophagy signaling in colorectal cancer. Cell Death Dis 12:449 (2021). PubMed: 33953166