Anti-ATF6抗体(ab37149)
Key features and details
- Rabbit polyclonal to ATF6
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ATF6抗体
参阅全部 ATF6 一抗 -
描述
兔多克隆抗体to ATF6 -
宿主
Rabbit -
特异性
Based on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as it recognizes a peptide sequence from near the amino terminus. We have not tested stressed cells to see if the 50kDa cleavage product can be detected.
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经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human ATF6 (N terminal). 17 a.a. immunogen somewhere between a.a. 25-75
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阳性对照
- WB: Human breast cell lysate. Mouse heart and skeletal lysates. Rat kidney lysate. ICC: HeLa and mouse reticulum sarcoma cells. IHC-P: Mouse and rat brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Immunoaffinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37149于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) |
Use a concentration of 10 µg/ml.
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| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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ICC
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
结构域
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. -
翻译后修饰
During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK. -
细胞定位
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 22926 Human
- Entrez Gene: 226641 Mouse
- Entrez Gene: 304962 Rat
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 492740 Human
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别名
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
图片
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Western blot - Anti-ATF6 antibody (ab37149)All lanes : Anti-ATF6 antibody (ab37149) at 1 µg/ml
Lane 1 : Mouse Heart Lysate
Lane 2 : Mouse Skeletal Muscle Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP Conjugate at 1/10000 dilution
Predicted band size: 75 kDaBlocking buffer: 5% NFDM/TBST.
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Western blot - Anti-ATF6 antibody (ab37149)Anti-ATF6 antibody (ab37149) at 1 µg/ml + Rat Kidney Lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP Conjugate at 1/10000 dilution
Predicted band size: 75 kDaBlocking buffer: 5% NFDM/TBST.
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Immunocytochemistry - Anti-ATF6 antibody (ab37149)This image is courtesy of an Abreview submitted by Dr Dan Tattersallab37149 staining human HeLa cells by ICC. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit ab175471 diluted at 1/1000.
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Immunocytochemistry - Anti-ATF6 antibody (ab37149)This image is courtesy of an anonymous Abreview.HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ATF6 (green) using ab37149 at 1/100 dilution in ICC, followed by Goat anti-rabbit Alexa Fluor® 488.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF6 antibody (ab37149)Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab37149 at 5µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as a secondary. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF6 antibody (ab37149)Immunohistochemical analysis of paraffin-embedded rat brain tissue using ab37149 at 5µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as a secondary. Counter stained with Hematoxylin.
数据表及文件
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Datasheet download
文献 (87)
ab37149 被引用在 87 文献中.
- Li S et al. Sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA2b) mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain. Br J Pharmacol 179:2016-2036 (2022). PubMed: 34811737
- Zheng C et al. Analyze Mouse Knockout Models of UPR Pathway Elements. Methods Mol Biol 2378:205-219 (2022). PubMed: 34985702
- Fan K et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev 2022:5652586 (2022). PubMed: 35368865
- Lu X et al. TSG-6 released from adipose stem cells-derived small extracellular vesicle protects against spinal cord ischemia reperfusion injury by inhibiting endoplasmic reticulum stress. Stem Cell Res Ther 13:291 (2022). PubMed: 35831906
- Li S et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
