Anti-ATF6抗体[1-7] (ab122897)
![Western blot - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.cn/ps/products/122/ab122897/Images/ab122897-275675-anti-atf6-antibody-1-7-western-blot.jpg "Western blot - Anti-ATF6 antibody [1-7] (ab122897)")
Key features and details
- Mouse monoclonal [1-7] to ATF6
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-ATF6抗体[1-7]
参阅全部 ATF6 一抗 -
描述
小鼠单克隆抗体[1-7] to ATF6 -
宿主
Mouse -
特异性
specific to human ATF6 alfa no cross reactivity with mouse ATF6 alfa. -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Recombinant fragment (His-tag) corresponding to Human ATF6 (N terminal). Epitope is not determined
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阳性对照
- 293T, HeLa S3 Tet-off, 293 and HeLa cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
纯化说明
ab122897 was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes, then filter sterilized. -
克隆
单克隆 -
克隆编号
1-7 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122897于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) |
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
If clear result not obtained, immunoprecipitation may help. |
| IP |
Use at an assay dependent concentration.
|
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| ICC/IF | (4) |
1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa. If clear result not obtained, immunoprecipitation may help. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
1/100. |
靶标
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功能
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
结构域
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. -
翻译后修饰
During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK. -
细胞定位
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 22926 Human
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 492740 Human
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别名
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
图片
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Western blot - Anti-ATF6 antibody [1-7] (ab122897)
Lane 1: Wild type HAP1 whole cell lysate (50 µg)
Lane 2: ATF6 knockout HAP1 whole cell lysate (50 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab122897 observed at 95 kDa. Red - loading control, ab181602, observed at 37 kDa.ab122897 was shown to specifically recact with ATF6 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ATF6 knockout samples were used. Wild-type and ATF6 knockout samples were subjected to SDS-PAGE. Ab122897 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.cn/ps/products/122/ab122897/Images/ab122897-253198-anti-atf6-antibody-1-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody [1-7] (ab122897)Immunofluorescent analysis of HeLa cells using ab122897 at a dilution of 1/100. The cells were fixed with paraformaldehyde. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG at a dilution of 1/1000. The antibody staining is shown in the top panel, and the merged image with DAPI counter-staining is shown in the lower panel.
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![Western blot - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.cn/ps/products/122/ab122897/Images/ab122897-136203-anti-atf6-antibody-1-7-western-blot.jpg)
Western blot - Anti-ATF6 antibody [1-7] (ab122897)All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution
Lane 1 : 293T cell lysate: control
Lane 2 : 293T cell lysate: treated with 2 µg/ml tunicamycin for 7 hours
Lane 3 : 293T cell lysate: treated with 1mM DTT for 1 hour.
Lane 4 : HeLa S3 Tet-off cell lysate: control
Lane 5 : HeLa S3 Tet-off cell lysate: treated with 1mM DTT for 1 hour.
Predicted band size: 75 kDa
Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells. -
![Western blot - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.cn/ps/products/122/ab122897/Images/ab122897-136202-anti-atf6-antibody-1-7-western-blot.jpg)
Western blot - Anti-ATF6 antibody [1-7] (ab122897)All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution
Lane 1 : HeLa cell extract: control
Lane 2 : HeLa cell extract: treated with 1mM DTT for 1 hour
Lane 3 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 3 hour
Lane 4 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 7 hour
Lane 5 : 293 cell extract: control
Lane 6 : 293 cell extract: treated with 1mM DTT for 1 hour
Lane 7 : 293 cell extract: treated with 2 µg/ml tunicamycin for 3 hour
Lane 8 : 293 cell extract: treated with 2 µg/ml tunicamycin for 7 hour
Predicted band size: 75 kDa
Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells. -
![Immunoprecipitation - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.cn/ps/products/122/ab122897/Images/ab122897-136201-anti-atf6-antibody-1-7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ATF6 antibody [1-7] (ab122897)ATF6 was detected by Western blot (Input; lanes 1, 2, 7, and 8) using ab122897 (No. 1-7). After immunoprecipitation (IP) with non-related IgG (IP; lanes 3, 4, 9, and 10) or ab122897 (No. 1-7) (IP; lanes 5, 6, 11, and 12), samples were subjected to SDS-PAGE and analyzed by Western blot using ab122897 (No. 1-7) and anti-mouse IgG antibody (light chain specific).
数据表及文件
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SDS download
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Datasheet download
文献 (70)
ab122897 被引用在 70 文献中.
- Shi D et al. Autophagy is induced by swine acute diarrhea syndrome coronavirus through the cellular IRE1-JNK-Beclin 1 signaling pathway after an interaction of viral membrane-associated papain-like protease and GRP78. PLoS Pathog 19:e1011201 (2023). PubMed: 36888569
- Woodley K et al. Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells to therapy by driving ferroptotic cell death. Nat Commun 14:2132 (2023). PubMed: 37059720
- Arabkari V et al. UPR-Induced miR-616 Inhibits Human Breast Cancer Cell Growth and Migration by Targeting c-MYC. Int J Mol Sci 24:N/A (2023). PubMed: 37685841
- Deng J et al. Human-induced pluripotent stem cell-derived cerebral organoid of leukoencephalopathy with vanishing white matter. CNS Neurosci Ther 29:1049-1066 (2023). PubMed: 36650674
- Sun S et al. ER stress activates TAp73α to promote colon cancer cell apoptosis via the PERK-ATF4 pathway. J Cancer 14:1946-1955 (2023). PubMed: 37497416