AB278689

重组Anti-ATF2 (phospho T71)抗体[ATF2T71-G3]

Anti-ATF2 (phospho T71) antibody [ATF2T71-G3]

20ul selling size
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ATF2 phospho T71 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATF2 phospho T71.

查看别名

CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2

5 Images

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) using ab278689 at 0.1µg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using ab278689.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using ab278689 at 0.1µg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using ab278689 at 0.1μg/mL.

Supplier Data

Western blot - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

All lanes:

Western blot - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689) at 0.1 µg/mL

Lane 1:

HEK-293T cell extract untreated

Lane 2:

HEK-293T cell extract treated with UV

Predicted band size: 55 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

ATF2T71-G3

亚型

IgG

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Human

应用

Flow Cyt, WB

applications

免疫原

Synthetic Peptide within Human ATF2 phospho T71. The exact immunogen used to generate this antibody is proprietary information.

P15336

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.

Biological function summary

ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.

Pathways

ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.

ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed : 10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

See full target information ATF2 phospho T71

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:122 PubMed40158101

2025

The m6A reader IGF2BP2 promotes pancreatic cancer progression through the m6A-SLC1A5-mTORC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Pu,Yuting Wu,Weiguo Long,Xinyu Sun,Xiao Yuan,Deqiang Wang,Xu Wang,Min Xu

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-ATF2 (phospho T71)抗体[ATF2T71-G3]

Anti-ATF2 (phospho T71) antibody [ATF2T71-G3]

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

Western blot - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (AB278689)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原