Anti-ATF2 (phospho S62 + S44)抗体(ab28847)
Key features and details
- Rabbit polyclonal to ATF2 (phospho S62 + S44)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ATF2 (phospho S62 + S44)抗体
参阅全部 ATF2 一抗 -
描述
兔多克隆抗体to ATF2 (phospho S62 + S44) -
宿主
Rabbit -
特异性
ab28847 recognises Phospho-ATF-2(Ser44) -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ATF2 (phospho S44 + S62).
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阳性对照
- HeLa lysate; Breast carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28847于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
靶标
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功能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
组织特异性
Abundant expression seen in the brain. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻译后修饰
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
see all -
别名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
图片
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Western blot - Anti-ATF2 (phospho S62 + S44) antibody (ab28847)All lanes : Anti-ATF2 (phospho S62 + S44) antibody (ab28847)
Lane 1 : HeLa cells treated with TNF-alpha with phospho peptide
Lane 2 : HeLa cells treated with TNF-alpha
Predicted band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 (phospho S62 + S44) antibody (ab28847)Immunohistochemistry (Paraffin-embedded sections) analysis of human breast carcinoma labeling ATF2 with ab28847.
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Western blot - Anti-ATF2 (phospho S62 + S44) antibody (ab28847)All lanes : Anti-ATF2 (phospho S62 + S44) antibody (ab28847)
Lane 1 : Hela Lysate with Immunizing peptide
Lane 2 : Hela Lysate
Predicted band size: 52 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab28847 尚未被引用在任何文献中。